Visualization through magnetic resonance imaging of DNA internalized following "In vivo" electroporation

Simonetta Geninatti Crich; Stefania Lanzardo; Alessandro Barge; Giovanna Esposito; Lorenzo Tei; Guido Forni; Silvio Aime

doi:10.1162/15353500200504151

Visualization through magnetic resonance imaging of DNA internalized following "In vivo" electroporation

Simonetta Geninatti Crich, Stefania Lanzardo, Alessandro Barge, Giovanna Esposito, Lorenzo Tei, Guido Forni, Silvio Aime

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The ability to visualize plasmid DNA entrapment in muscle cells undergoing an "in vivo" electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd-DOTA-spd complex as imaging reporter. Gd-DOTA-spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, K_a = 2.2 × 10³ M^-1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.

Lingua originale	Inglese
pagine (da-a)	7-17
Numero di pagine	11
Rivista	Molecular Imaging
Volume	4
Numero di pubblicazione	1
DOI	https://doi.org/10.1162/15353500200504151
Stato di pubblicazione	Pubblicato - 2005
Pubblicato esternamente	Sì

Accesso al documento

10.1162/15353500200504151

Altri file e collegamenti

Link to publication in Scopus

Cita questo

@article{923368bc621f4caba8baa346ebf48b74,

title = "Visualization through magnetic resonance imaging of DNA internalized following {"}In vivo{"} electroporation",

abstract = "The ability to visualize plasmid DNA entrapment in muscle cells undergoing an {"}in vivo{"} electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd-DOTA-spd complex as imaging reporter. Gd-DOTA-spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, Ka = 2.2 × 103 M-1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.",

keywords = "DNA, Electroporation, Gd(III) complex, MRI, Transfection",

author = "Crich, {Simonetta Geninatti} and Stefania Lanzardo and Alessandro Barge and Giovanna Esposito and Lorenzo Tei and Guido Forni and Silvio Aime",

year = "2005",

doi = "10.1162/15353500200504151",

language = "English",

volume = "4",

pages = "7--17",

journal = "Molecular Imaging",

issn = "1535-3508",

publisher = "SAGE Publications Inc.",

number = "1",

}

TY - JOUR

T1 - Visualization through magnetic resonance imaging of DNA internalized following "In vivo" electroporation

AU - Crich, Simonetta Geninatti

AU - Lanzardo, Stefania

AU - Barge, Alessandro

AU - Esposito, Giovanna

AU - Tei, Lorenzo

AU - Forni, Guido

AU - Aime, Silvio

PY - 2005

Y1 - 2005

N2 - The ability to visualize plasmid DNA entrapment in muscle cells undergoing an "in vivo" electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd-DOTA-spd complex as imaging reporter. Gd-DOTA-spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, Ka = 2.2 × 103 M-1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.

AB - The ability to visualize plasmid DNA entrapment in muscle cells undergoing an "in vivo" electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd-DOTA-spd complex as imaging reporter. Gd-DOTA-spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, Ka = 2.2 × 103 M-1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.

KW - DNA

KW - Electroporation

KW - Gd(III) complex

KW - MRI

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=26944449754&partnerID=8YFLogxK

U2 - 10.1162/15353500200504151

DO - 10.1162/15353500200504151

M3 - Article

SN - 1535-3508

VL - 4

SP - 7

EP - 17

JO - Molecular Imaging

JF - Molecular Imaging

IS - 1

ER -

Visualization through magnetic resonance imaging of DNA internalized following "In vivo" electroporation

Abstract

Accesso al documento

Altri file e collegamenti

Fingerprint

Cita questo