Variations in chromatin structure in host nuclei of a vesicular arbuscular mycorrhiza

G. BERTA, S. SGORBATI, V. SOLER, A. FUSCONI, A. TROTTA, A. CITTERIO, M. G. BOTTONE, E. SPARVOLI, S. SCANNERINI

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Microfluorimetric and flow cytometric analyses were used in nuclei of cortical cells of roots in the endomycorrhizal system Allium porrum L. + a Glomus sp., strain E3 (from Rothamsted Station), in an attempt to explain nuclear hypertrophy. No variation of DNA content in comparison with the controls was observed in mycorrhizal roots. An increase of fluorescence (about 25 %) was observed in mycorrhizal root nuclei stained with an undersaturating concentration of DAPI. This can be explained by a greater accessibility of DNA to fluorochromes owing to a lower degree of chromatin condensation, as confirmed by ultrastructural data. Our results suggest a possible explanation of nuclear hypertrophy and, further, show that variations of chromatin condensation can occur in mature plant cell nuclei, even though this, in differentiated plant cells, is generally considered to be independent of the cellular functions.

Lingua originaleInglese
pagine (da-a)199-205
Numero di pagine7
RivistaNew Phytologist
Volume114
Numero di pubblicazione2
DOI
Stato di pubblicazionePubblicato - feb 1990
Pubblicato esternamente

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