TY - JOUR
T1 - Validation of the 13C-urea breath test for the diagnosis of Helicobacter pylori infection in children
T2 - A multicenter study
AU - Bazzoli, Franco
AU - Cecchini, Liliana
AU - Corvaglia, Luigi
AU - Dall'Antonia, Martino
AU - De Giacomo, Costantino
AU - Fossi, Stefania
AU - Casali, Luigi Gobio
AU - Gullini, Sergio
AU - Lazzari, Rosanna
AU - Leggeri, Giorgio
AU - Lerro, Pietro
AU - Valdambrini, Valeria
AU - Mandrioli, Gianni
AU - Marani, Miris
AU - Martelli, Paolo
AU - Miano, Angelo
AU - Nicolini, Gianni
AU - Oderda, Giuseppina
AU - Pazzi, Paolo
AU - Pozzato, Paolo
AU - Ricciardiello, Luigi
AU - Roda, Enrico
AU - Simoni, Patrizia
AU - Sottili, Sandra
AU - Torre, Giuliano
AU - Urso, Luigina
AU - Zagari, Rocco Maurizio
PY - 2000/3
Y1 - 2000/3
N2 - OBJECTIVE: The 13C-urea breath test (13C-UBT) is a safe, noninvasive, and accurate test for the detection of Helicobacter pylori (H. pylori) infection in adults. The aim of this study was to evaluate sensitivity and specificity of 13C-UBT in children using different types of test meal, doses of 13C-urea and breath sampling intervals. As yet, a validated, standardized 13C-UBT protocol for children has not been formulated. METHODS: 13C-UBT was performed in 115 children and repeated within 3 days, modifying the test meal or the dose of 13C-urea. H. pylori status was assessed by histology and rapid urease test. 13C-UBT was performed using 100 mg or 50 mg of 13C-urea and a fatty test meal (100 FA; 50 FA), 50 mg of 13C-urea, and a carbohydrate test meal (50 CA). Breath samples were collected every 10 min for 60 min. RESULTS: The 13C-UBT in children was highly sensitive and specific with all three protocols used. The best combination of sensitivity (97.92%) and specificity (97.96%) was obtained with Protocol 50 FA at 30 min with a cut-off of 3.5 per mil. CONCLUSIONS: The 13C-UBT is an accurate test for the detection of H. pylori infection also in children. Administration of 50 mg of 13C-urea, a fatty test meal, and breath sampling at 30 min appears to be the most convenient protocol. (C) 2000 by Am. Coll. of Gastroenterology.
AB - OBJECTIVE: The 13C-urea breath test (13C-UBT) is a safe, noninvasive, and accurate test for the detection of Helicobacter pylori (H. pylori) infection in adults. The aim of this study was to evaluate sensitivity and specificity of 13C-UBT in children using different types of test meal, doses of 13C-urea and breath sampling intervals. As yet, a validated, standardized 13C-UBT protocol for children has not been formulated. METHODS: 13C-UBT was performed in 115 children and repeated within 3 days, modifying the test meal or the dose of 13C-urea. H. pylori status was assessed by histology and rapid urease test. 13C-UBT was performed using 100 mg or 50 mg of 13C-urea and a fatty test meal (100 FA; 50 FA), 50 mg of 13C-urea, and a carbohydrate test meal (50 CA). Breath samples were collected every 10 min for 60 min. RESULTS: The 13C-UBT in children was highly sensitive and specific with all three protocols used. The best combination of sensitivity (97.92%) and specificity (97.96%) was obtained with Protocol 50 FA at 30 min with a cut-off of 3.5 per mil. CONCLUSIONS: The 13C-UBT is an accurate test for the detection of H. pylori infection also in children. Administration of 50 mg of 13C-urea, a fatty test meal, and breath sampling at 30 min appears to be the most convenient protocol. (C) 2000 by Am. Coll. of Gastroenterology.
UR - https://www.scopus.com/pages/publications/0033621996
U2 - 10.1016/S0002-9270(99)00895-3
DO - 10.1016/S0002-9270(99)00895-3
M3 - Article
SN - 0002-9270
VL - 95
SP - 646
EP - 650
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
IS - 3
ER -