TY - JOUR
T1 - Urocortin II induces nitric oxide production through cAMP and Ca2+ related pathways in endothelial cells
AU - GROSSINI, Elena
AU - MOLINARI, Claudio Giuseppe
AU - DA, MARY
AU - UBERTI, FRANCESCA
AU - RIBICHINI, F
AU - PP, CAIMMI
AU - VACCA, Giovanni
PY - 2009
Y1 - 2009
N2 - BACKGROUND: Urocortin II has previously been shown in anesthetized pigs to increase coronary blood flow through activation of the endothelial nitric oxide synthase (eNOS) pathway and involvement of the subtype 2 of corticotropin releasing factor receptors (CRFR2). However, little information has been available regarding the intracellular signalling through these receptors and leading to the release of nitric oxide (NO).
AIM:
The present study was therefore planned to determine the mechanism involved in such signalling.
METHODS:
In porcine aortic endothelial cells (PAE) the effects of urocortin II on NO production and ERK, Akt, p38 and eNOS phosphorylation were examined in absence or presence of the adenylyl cyclase agonist forskolin and antagonist 2'5' dideoxyadenosine, the Ca2+ ionophore A23187, the Ca2+-calmodulin-kinase inhibitor KN93, the CRFR2 blocker astressin 2B and of the protein kinases specific inhibitors UO126, wortmannin and SB203580.
RESULTS:
Urocortin II caused a significant increase of NO production, which was amplified by forskolin and A23187 (P <0.05). All effects of urocortin II were abolished by l-NAME, 2'5' dideoxyadenosine, KN93, astressin 2B and by pre-treatment of cells with UO126, wortmannin and SB203580. Western Blot analysis confirmed the involvement of ERK, Akt and p38 in the eNOS activation.
CONCLUSION:
In PAE urocortin II interaction with CRFR2 caused a cAMP-dependent and Ca2+-related phoshorylation of ERK, Akt and p38 leading to eNOS activation.
AB - BACKGROUND: Urocortin II has previously been shown in anesthetized pigs to increase coronary blood flow through activation of the endothelial nitric oxide synthase (eNOS) pathway and involvement of the subtype 2 of corticotropin releasing factor receptors (CRFR2). However, little information has been available regarding the intracellular signalling through these receptors and leading to the release of nitric oxide (NO).
AIM:
The present study was therefore planned to determine the mechanism involved in such signalling.
METHODS:
In porcine aortic endothelial cells (PAE) the effects of urocortin II on NO production and ERK, Akt, p38 and eNOS phosphorylation were examined in absence or presence of the adenylyl cyclase agonist forskolin and antagonist 2'5' dideoxyadenosine, the Ca2+ ionophore A23187, the Ca2+-calmodulin-kinase inhibitor KN93, the CRFR2 blocker astressin 2B and of the protein kinases specific inhibitors UO126, wortmannin and SB203580.
RESULTS:
Urocortin II caused a significant increase of NO production, which was amplified by forskolin and A23187 (P <0.05). All effects of urocortin II were abolished by l-NAME, 2'5' dideoxyadenosine, KN93, astressin 2B and by pre-treatment of cells with UO126, wortmannin and SB203580. Western Blot analysis confirmed the involvement of ERK, Akt and p38 in the eNOS activation.
CONCLUSION:
In PAE urocortin II interaction with CRFR2 caused a cAMP-dependent and Ca2+-related phoshorylation of ERK, Akt and p38 leading to eNOS activation.
KW - metabolism, Nitric Oxide
KW - pharmacology, Coronary Circulation
KW - urocortin
KW - metabolism, Nitric Oxide
KW - pharmacology, Coronary Circulation
KW - urocortin
UR - https://iris.uniupo.it/handle/11579/29907
U2 - 10.1159/000204097
DO - 10.1159/000204097
M3 - Article
SN - 1015-8987
VL - 23
SP - 87
EP - 96
JO - Cellular Physiology and Biochemistry
JF - Cellular Physiology and Biochemistry
IS - 1-3
ER -