TY - JOUR
T1 - Uptake of antitumor platinum(II)-complexes by cancer cells, assayed by inductively coupled plasma mass spectrometry (ICP-MS)
AU - Ghezzi, Anna Rita
AU - Aceto, Maurizio
AU - Cassino, Claudio
AU - Gabano, Elisabetta
AU - Osella, Domenico
N1 - Funding Information:
This work was financially supported by MIUR (Roma) within PRIN2001 project. We are indebted to Johnson and Matthey (Reading, UK) for a generous loan of K 2 PtCl 4 . AR.G. thanks CIRCMSB (Bari) for research fellowship. This research was carried out in the frame of the European Cooperation Cost D20 action (Metal compounds in the treatment of cancer and viral diseases) and Cost B16 action (Multidrug resistance reversal).
PY - 2004/1
Y1 - 2004/1
N2 - A systematic study on intracellular Pt uptake and Pt accumulation ratio in breast cancer MCF-7 cell line has been performed on a number of Pt(II)-complexes, namely cisplatin, carboplatin and oxaliplatin, clinically employed as antitumor drugs, trans- and cis-[Pt(Cl)2(pyridine) 2] and cis-[Pt(Cl)2(pyridine)(5-SO3H- isoquinoline)] complexes, previously investigated also as potential telomerase inhibitors. In particular, long incubation times have been chosen in order to understand the fate of the complexes in the cells. For this purpose, sub-acute drug concentrations must be employed and, therefore, a very sensitive method of analysis like as inductively coupled plasma mass spectrometry (ICP-MS) superior to the widely employed atomic absorption spectroscopy (AAS) has been adopted. Any relationships among uptake/accumulation and several parameters such as drug structure, lipophilicity, drug concentration and incubation time have been sought and analyzed: the bulk of data point for a passive diffusion mechanism through the cell membrane.
AB - A systematic study on intracellular Pt uptake and Pt accumulation ratio in breast cancer MCF-7 cell line has been performed on a number of Pt(II)-complexes, namely cisplatin, carboplatin and oxaliplatin, clinically employed as antitumor drugs, trans- and cis-[Pt(Cl)2(pyridine) 2] and cis-[Pt(Cl)2(pyridine)(5-SO3H- isoquinoline)] complexes, previously investigated also as potential telomerase inhibitors. In particular, long incubation times have been chosen in order to understand the fate of the complexes in the cells. For this purpose, sub-acute drug concentrations must be employed and, therefore, a very sensitive method of analysis like as inductively coupled plasma mass spectrometry (ICP-MS) superior to the widely employed atomic absorption spectroscopy (AAS) has been adopted. Any relationships among uptake/accumulation and several parameters such as drug structure, lipophilicity, drug concentration and incubation time have been sought and analyzed: the bulk of data point for a passive diffusion mechanism through the cell membrane.
KW - Antitumor chemotherapy
KW - Cisplatin-like complexes
KW - ICP-MS
KW - Platinum uptake
UR - http://www.scopus.com/inward/record.url?scp=0344255614&partnerID=8YFLogxK
U2 - 10.1016/j.jinorgbio.2003.08.014
DO - 10.1016/j.jinorgbio.2003.08.014
M3 - Article
SN - 0162-0134
VL - 98
SP - 73
EP - 78
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 1
ER -
