@inbook{a806e43124614db786a540c157efdcfb,
title = "Two-Dimensional Gel Electrophoresis Image Analysis",
abstract = "Gel-based proteomics is still quite widespread due to its high-resolution power; the experimental approach is based on differential analysis, where groups of samples (e.g., control vs diseased) are compared to identify panels of potential biomarkers. However, the reliability of the result of the differential analysis is deeply influenced by 2D-PAGE maps image analysis procedures. The analysis of 2D-PAGE images consists of several steps, such as image preprocessing, spot detection and quantitation, image warping and alignment, spot matching. Several approaches are present in literature, and classical or last-generation commercial software packages exploit different algorithms for each step of the analysis. Here, the most widespread approaches and a comparison of the different strategies are presented.",
keywords = "2D-PAGE, Image analysis, Image preprocessing, Spot detection and quantification, Spot matching, Warping",
author = "Elisa Robotti and Elisa Cal{\`a} and Emilio Marengo",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1641-3_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "3--13",
booktitle = "Methods in Molecular Biology",
}