Thrombin induces the association of cyclic ADP-ribose-synthesizing CD38 with the platelet cytoskeleton

Mauro Torti, Enrico Tolnai Festetics, Alessandra Bertoni, Fabiola Sinigaglia, Cesare Balduini

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

The effect of platelet stimulation on the subcellular localization of CD38, a membrane glycoprotein that catalyses the synthesis of cyclic ADP- ribose from β-NAD+ was investigated. Treatment of human platelets with thrombin caused the association of about 40% of the total ADP-ribosyl cyclase activity with the cytoskeleton, through the translocation of the CD38 molecule from the Triton X-100-soluble to the insoluble fraction. The interaction of CD38 with the cytoskeleton was a specific and reversible process, mediated by the binding to the actin-rich filaments and was inhibited by treatment of platelets with cytochalasin D. This event was regulated by integrin α(11b)β3 and platelet aggregation as it was prevented by the inhibition of fibrinogen binding and was not obeserved in platelets from a patient affected by Glanzmann thrombasthenia. These results demonstrate that the subcellular localization of CD38 can be influenced by platelet stimulation with physiological agonists, and that membrane CD38 can interact with intracellular proteins.

Lingua originaleInglese
pagine (da-a)200-204
Numero di pagine5
RivistaFEBS Letters
Volume428
Numero di pubblicazione3
DOI
Stato di pubblicazionePubblicato - 29 mag 1998
Pubblicato esternamente

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