The use of legendre and zernike moment functions for the comparison of 2-D PAGE maps

Emilio Marengo, Elisa Robotti, Marco Demartini

Risultato della ricerca: Capitolo in libro/report/atti di convegnoContributo in volume (Capitolo o Saggio)peer review

Abstract

The comparison of 2-D maps is not trivial, the main difficulties being the high complexity of the sample and the large experimental variability characterizing 2-D gel electrophoresis. The comparison of maps from control and treated samples is usually performed by specific software, providing the so-called spot volume dataset where each spot of a specific map is matched to its analogous in other maps, and they are described by their optical density, which is supposed to be related to the underlying protein amount. Here, a different approach is presented, based on the direct comparison of 2-D map images: each map is decomposed in terms of moment functions, successively applying the multivariate tools usually adopted in image analysis problems. The moments calculated are then treated with multivariate classification techniques. Here, two types of moment functions are presented (Legendre and Zernike moments), while linear discriminant analysis and partial least squares discriminant analysis are exploited as classification tools to provide the classification of the samples. The procedure is applied to a sample dataset to prove its effectiveness.

Lingua originaleInglese
Titolo della pubblicazione ospiteMethods in Molecular Biology
EditoreHumana Press Inc.
Pagine271-288
Numero di pagine18
DOI
Stato di pubblicazionePubblicato - 2016

Serie di pubblicazioni

NomeMethods in Molecular Biology
Volume1384
ISSN (stampa)1064-3745

Fingerprint

Entra nei temi di ricerca di 'The use of legendre and zernike moment functions for the comparison of 2-D PAGE maps'. Insieme formano una fingerprint unica.

Cita questo