TY - JOUR
T1 - The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula
T2 - A practical approach
AU - Cajaraville, Miren P.
AU - Bebianno, Maria J.
AU - Blasco, Julián
AU - Porte, Cinta
AU - Sarasquete, Carmen
AU - Viarengo, Aldo
N1 - Funding Information:
This collaborative effort has been possible thanks to the funding of CICYT (Spanish Ministry of Education and Culture) through a Special Action (ref AMB98-1330-E). Work in the laboratory of MP Cajaraville has been funded by projects AMB96-0523 (CICYT), UPV 076.310-EC219/97 (University of the Basque Country) and PL97-3827 (EU FAIR Programme). The work in the laboratory of C. Porte and A. Viarengo was partially supported by the CE-BIOMAR project refs. EV5V-CT94-0550 and ENV4-CT96-0300.
PY - 2000/3/20
Y1 - 2000/3/20
N2 - Within the frame of the 2nd Iberian Congress of Environmental Contamination and Toxicology (University of the Basque Country, Leioa, June 1998) a workshop was held about the use of biomarkers in marine pollution monitoring. Among others, the following biomarkers received special attention: metallothionein induction, acetylcholinesterase inhibition, cytochrome P450 system induction, imposex, lysosomal enlargement and lysosomal membrane destabilisation, and peroxisome proliferation. These biomarkers can be used to evaluate exposure to and effect of different contaminants (metals, organic xenobiotics and organometallic compounds) and they can be measured using different methodological approaches (biochemistry, cytochemistry, immunochemical methods based on the use of biotechnology). Before the application of a set of biomarkers in pollution monitoring programmes, well-defined protocols of Quality Assurance have to be established to allow adequate comparison of results. It is also necessary to include analysis of standard reference materials and to obtain detailed knowledge of basal values and seasonal variations of the biomarkers in various species, as well as to integrate the information obtained with the different biomarkers. Marine bivalve molluscs such as mussels are appropriate sentinel species for most of the biomarkers proposed except for the induction of the cytochrome P450 system, which should be measured in fish, and the degree of imposex, which is a biomarker of exposure to TBT specifically measured in certain gastropod molluscs. As a result of the workshop, a battery of biomarkers of contaminant exposure and effects are proposed that could be incorporated into programmes monitoring the quality of the coastal environment in the Iberian Peninsula. These measures would be undertaken in conjunction with chemical measures of contaminant burdens in selected sentinel species. Copyright (C) 2000 Elsevier Science B.V.
AB - Within the frame of the 2nd Iberian Congress of Environmental Contamination and Toxicology (University of the Basque Country, Leioa, June 1998) a workshop was held about the use of biomarkers in marine pollution monitoring. Among others, the following biomarkers received special attention: metallothionein induction, acetylcholinesterase inhibition, cytochrome P450 system induction, imposex, lysosomal enlargement and lysosomal membrane destabilisation, and peroxisome proliferation. These biomarkers can be used to evaluate exposure to and effect of different contaminants (metals, organic xenobiotics and organometallic compounds) and they can be measured using different methodological approaches (biochemistry, cytochemistry, immunochemical methods based on the use of biotechnology). Before the application of a set of biomarkers in pollution monitoring programmes, well-defined protocols of Quality Assurance have to be established to allow adequate comparison of results. It is also necessary to include analysis of standard reference materials and to obtain detailed knowledge of basal values and seasonal variations of the biomarkers in various species, as well as to integrate the information obtained with the different biomarkers. Marine bivalve molluscs such as mussels are appropriate sentinel species for most of the biomarkers proposed except for the induction of the cytochrome P450 system, which should be measured in fish, and the degree of imposex, which is a biomarker of exposure to TBT specifically measured in certain gastropod molluscs. As a result of the workshop, a battery of biomarkers of contaminant exposure and effects are proposed that could be incorporated into programmes monitoring the quality of the coastal environment in the Iberian Peninsula. These measures would be undertaken in conjunction with chemical measures of contaminant burdens in selected sentinel species. Copyright (C) 2000 Elsevier Science B.V.
KW - Acetylcholinesterase
KW - Biomarkers
KW - Cytochrome P450 system
KW - Environmental pollution assessment
KW - Iberian Peninsula
KW - Imposex
KW - Lysosomes
KW - Metallothioneins
KW - Peroxisomes
KW - Stress enzymes
UR - http://www.scopus.com/inward/record.url?scp=0034689675&partnerID=8YFLogxK
U2 - 10.1016/S0048-9697(99)00499-4
DO - 10.1016/S0048-9697(99)00499-4
M3 - Article
SN - 0048-9697
VL - 247
SP - 295
EP - 311
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 2-3
ER -
