TY - JOUR
T1 - The transcriptomic analysis of nsc-34 motor neuron-like cells reveals that cannabigerol influences synaptic pathways
T2 - A comparative study with cannabidiol
AU - Gugliandolo, Agnese
AU - Silvestro, Serena
AU - Chiricosta, Luigi
AU - Pollastro, Federica
AU - Bramanti, Placido
AU - Mazzon, Emanuela
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10
Y1 - 2020/10
N2 - More than 120 cannabinoids were isolated from Cannabis sativa. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD.
AB - More than 120 cannabinoids were isolated from Cannabis sativa. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD.
KW - Cannabidiol
KW - Cannabigerol
KW - GABAergic synapses
KW - Glutamatergic synapses
KW - Motor neuron-like cells
KW - Transcriptomic analysis
UR - http://www.scopus.com/inward/record.url?scp=85092087485&partnerID=8YFLogxK
U2 - 10.3390/life10100227
DO - 10.3390/life10100227
M3 - Article
SN - 2075-1729
VL - 10
SP - 1
EP - 18
JO - Life
JF - Life
IS - 10
M1 - 227
ER -