The role of ph of the mobile-phase in ion-interaction RP-HPLC

M. C. Gennaro; D. Giacosa; C. Abrigo

doi:10.1080/10826079408013623

The role of ph of the mobile-phase in ion-interaction RP-HPLC

M. C. Gennaro, D. Giacosa, C. Abrigo

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pk_a values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

Lingua originale	Inglese
pagine (da-a)	4365-4380
Numero di pagine	16
Rivista	Journal of Liquid Chromatography
Volume	17
Numero di pubblicazione	20
DOI	https://doi.org/10.1080/10826079408013623
Stato di pubblicazione	Pubblicato - 1 dic 1994
Pubblicato esternamente	Sì

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10.1080/10826079408013623

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abstract = "The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.",

author = "Gennaro, {M. C.} and D. Giacosa and C. Abrigo",

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AB - The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pka values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

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JF - Journal of Liquid Chromatography

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The role of ph of the mobile-phase in ion-interaction RP-HPLC

Abstract

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