@article{1672f4a825354734a95d591b1e05e24c,
title = "The PML nuclear bodies-associated protein TTRAP regulates ribosome biogenesis in nucleolar cavities upon proteasome inhibition",
abstract = "TRAF and TNF receptor-associated protein (TTRAP) is a multifunctional protein that can act in the nucleus as a 5′-tyrosyl DNA phosphodiesterase and in the cytoplasm as a regulator of cell signaling. In this paper we show that in response to proteasome inhibition TTRAP accumulates in nucleolar cavities in a promyelocytic leukemia protein-dependent manner. In the nucleolus, TTRAP contributes to control levels of ribosomal RNA precursor and processing intermediates, and this phenotype is independent from its 5′-tyrosyl DNA phosphodiesterase activity. Our findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress.",
keywords = "PML, TTRAP, nucleolus, proteasome inhibition, rRNA biogenesis",
author = "S. Vilotti and M. Biagioli and R. Foti and \{Dal Ferro\}, M. and Lavina, \{Z. Scotto\} and L. Collavin and \{Del Sal\}, G. and S. Zucchelli and S. Gustincich",
note = "Funding Information: Acknowledgements. We are indebted to all the members of the SG lab for thought-provoking discussions and to Cristina Leonesi for technical support. We thank KW Caldecott (University of Sussex, Brighton, UK) for mutant TTRAP E152A and D262A constructs, V De Laurenzi (University of Rome Tor Vergata, Rome, Italy) for anti-FLASH and anti-coilin antibodies, and E Buratti (ICGEB, Trieste, Italy) for anti-SC35 antibody. We also thank Rosanna Parlato for helpful discussions and sharing of unpublished data. We would also like to thank Micaela Grandolfo for technical support with confocal microscope and Silvano Piazza for insights into image analysis softwares. This work was supported by the Telethon Grant GGP06268, by the Giovanni Armenise-Harvard Foundation and by the Italian Institute of Technology.",
year = "2012",
month = mar,
doi = "10.1038/cdd.2011.118",
language = "English",
volume = "19",
pages = "488--500",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Springer Nature",
number = "3",
}