The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes. A study of the implications of oxidative stress in intact cells

H. Thor, M. T. Smith, P. Hartzell, G. Bellomo, S. A. Jewell, S. Orrenius

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

The cytotoxic effects of many quinones are thought to be mediated through their one-electron reduction to semiquinone radicals, which subsequently enter redox cycles with molecular oxygen to produce active oxygen species and oxidative stress. The two-electron reduction of quinones to diols, mediated by DT-diaphorase (NAD(P)H: (quinone-acceptor) oxidoreductase), may therefore represent a detoxifying pathway which protects the cell from the formation of these reactive intermediates. By using menadione (2-methyl-1,4-naphthoquinone) and isolated hepatocytes, the relative contribution of the two pathways to quinone metabolism has been studied and a protective role for DT-diaphorase demonstrated. Moreover, in the presence of cytotoxic concentrations of menadione rapid changes in intracellular thiol and Ca 2+ homeostasis were observed. These were associated with alterations in the surface structure of the haptocytes which may be an early indication of cytotoxicity.

Lingua originaleInglese
pagine (da-a)12419-12425
Numero di pagine7
RivistaJournal of Biological Chemistry
Volume257
Numero di pubblicazione20
Stato di pubblicazionePubblicato - 1982
Pubblicato esternamente

Fingerprint

Entra nei temi di ricerca di 'The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes. A study of the implications of oxidative stress in intact cells'. Insieme formano una fingerprint unica.

Cita questo