The interaction of SiO2 nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx

Carla Distasi; Marianna Dionisi; Federico Alessandro Ruffinatti; Alessandra Gilardino; Roberta Bardini; Susanna Antoniotti; Federico Catalano; Eleonora Bassino; Luca Munaron; Gianmario Martra; Davide Lovisolo

doi:10.2217/nnm-2018-0256

The interaction of SiO₂ nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx

Carla Distasi, Marianna Dionisi, Federico Alessandro Ruffinatti, Alessandra Gilardino, Roberta Bardini, Susanna Antoniotti, Federico Catalano, Eleonora Bassino, Luca Munaron, Gianmario Martra, Davide Lovisolo

Dipartimento di Scienze del Farmaco

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Aim: To clarify the mechanisms of interaction between SiO₂ nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO₂ NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca²⁺]_i increase. Conclusion: These findings suggest that SiO₂ NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

Lingua originale	Inglese
pagine (da-a)	575-594
Numero di pagine	20
Rivista	Nanomedicine
Volume	8
Numero di pubblicazione	1
DOI	https://doi.org/10.2217/nnm-2018-0256
Stato di pubblicazione	Pubblicato - mar 2019

Accesso al documento

10.2217/nnm-2018-0256

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@article{31149195b567435a92c6bc0a1001ebd1,

title = "The interaction of SiO2 nanoparticles with the neuronal cell membrane: Activation of ionic channels and calcium influx",

abstract = "Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials \& methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.",

keywords = "Cx channels, Panx channels, TRPV4 channels, calcium, free radicals, neuronal cells, patch clamp, silica nanoparticles, single channel",

author = "Carla Distasi and Marianna Dionisi and Ruffinatti, \{Federico Alessandro\} and Alessandra Gilardino and Roberta Bardini and Susanna Antoniotti and Federico Catalano and Eleonora Bassino and Luca Munaron and Gianmario Martra and Davide Lovisolo",

note = "Publisher Copyright: {\textcopyright} 2019 Future Medicine Ltd.",

year = "2019",

month = mar,

doi = "10.2217/nnm-2018-0256",

language = "English",

volume = "8",

pages = "575--594",

journal = "Nanomedicine",

issn = "1743-5889",

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TY - JOUR

T1 - The interaction of SiO2 nanoparticles with the neuronal cell membrane

T2 - Activation of ionic channels and calcium influx

AU - Distasi, Carla

AU - Dionisi, Marianna

AU - Ruffinatti, Federico Alessandro

AU - Gilardino, Alessandra

AU - Bardini, Roberta

AU - Antoniotti, Susanna

AU - Catalano, Federico

AU - Bassino, Eleonora

AU - Munaron, Luca

AU - Martra, Gianmario

AU - Lovisolo, Davide

PY - 2019/3

Y1 - 2019/3

N2 - Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

AB - Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

KW - Cx channels

KW - Panx channels

KW - TRPV4 channels

KW - calcium

KW - free radicals

KW - neuronal cells

KW - patch clamp

KW - silica nanoparticles

KW - single channel

UR - https://www.scopus.com/pages/publications/85062849455

U2 - 10.2217/nnm-2018-0256

DO - 10.2217/nnm-2018-0256

M3 - Article

SN - 1743-5889

VL - 8

SP - 575

EP - 594

JO - Nanomedicine

JF - Nanomedicine

IS - 1