The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos

Shang Cao; Heather Bendall; Geoffrey G. Hicks; Abudi Nashabi; Hitoshi Sakano; Yoichi Shinkai; Marisa Gariglio; Eugene M. Oltz; H. Earl Ruley

doi:10.1128/MCB.23.15.5301-5307.2003

The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos

Shang Cao, Heather Bendall, Geoffrey G. Hicks, Abudi Nashabi, Hitoshi Sakano, Yoichi Shinkai, Marisa Gariglio, Eugene M. Oltz, H. Earl Ruley

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.

Lingua originale	Inglese
pagine (da-a)	5301-5307
Numero di pagine	7
Rivista	Molecular and Cellular Biology
Volume	23
Numero di pubblicazione	15
DOI	https://doi.org/10.1128/MCB.23.15.5301-5307.2003
Stato di pubblicazione	Pubblicato - ago 2003
Pubblicato esternamente	Sì

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10.1128/MCB.23.15.5301-5307.2003

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title = "The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos",

abstract = "The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.",

author = "Shang Cao and Heather Bendall and Hicks, {Geoffrey G.} and Abudi Nashabi and Hitoshi Sakano and Yoichi Shinkai and Marisa Gariglio and Oltz, {Eugene M.} and Ruley, {H. Earl}",

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AU - Cao, Shang

AU - Bendall, Heather

AU - Hicks, Geoffrey G.

AU - Nashabi, Abudi

AU - Sakano, Hitoshi

AU - Shinkai, Yoichi

AU - Gariglio, Marisa

AU - Oltz, Eugene M.

AU - Ruley, H. Earl

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AB - The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.

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DO - 10.1128/MCB.23.15.5301-5307.2003

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The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos

Abstract

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