The expression of the PDZ protein MALS-1/Velis is regulated by calcium and calcineurin in cerebellar granule cells

Bastiano Sanna, Dana Kramer, Armando A. Genazzani

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Activity-dependent gene expression is thought to be important in shaping neuronal development and in modifying the protein content of neurons. Ca2+ entry into neurons appears to be one of the key effectors of activity-dependent gene expression. Among the possible downstream targets of calcium, the protein phosphatase calcineurin represents a prime candidate. We hereby report that in cultured cerebellar granule cells the activation of the Ca2+/calcineurin pathway via either voltage- or ligand-operated Ca2+ channels regulates MALS-1 and MALS-2 expression at the transcriptional level. These proteins are integral parts of the post-synaptic density and are also involved in receptor trafficking. MALS regulation is not at the level of mRNA stability and does not require de novo protein synthesis, thereby suggesting a direct pathway. These data suggest that Ca2+ entry by means of calcineurin is capable of controlling the structure of the post-synaptic density by controlling the expression of key components at the transcriptional level.

Lingua originaleInglese
pagine (da-a)49585-49590
Numero di pagine6
RivistaJournal of Biological Chemistry
Volume277
Numero di pubblicazione51
DOI
Stato di pubblicazionePubblicato - 20 dic 2002
Pubblicato esternamente

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