TY - JOUR
T1 - The expression of the PDZ protein MALS-1/Velis is regulated by calcium and calcineurin in cerebellar granule cells
AU - Sanna, Bastiano
AU - Kramer, Dana
AU - Genazzani, Armando A.
PY - 2002/12/20
Y1 - 2002/12/20
N2 - Activity-dependent gene expression is thought to be important in shaping neuronal development and in modifying the protein content of neurons. Ca2+ entry into neurons appears to be one of the key effectors of activity-dependent gene expression. Among the possible downstream targets of calcium, the protein phosphatase calcineurin represents a prime candidate. We hereby report that in cultured cerebellar granule cells the activation of the Ca2+/calcineurin pathway via either voltage- or ligand-operated Ca2+ channels regulates MALS-1 and MALS-2 expression at the transcriptional level. These proteins are integral parts of the post-synaptic density and are also involved in receptor trafficking. MALS regulation is not at the level of mRNA stability and does not require de novo protein synthesis, thereby suggesting a direct pathway. These data suggest that Ca2+ entry by means of calcineurin is capable of controlling the structure of the post-synaptic density by controlling the expression of key components at the transcriptional level.
AB - Activity-dependent gene expression is thought to be important in shaping neuronal development and in modifying the protein content of neurons. Ca2+ entry into neurons appears to be one of the key effectors of activity-dependent gene expression. Among the possible downstream targets of calcium, the protein phosphatase calcineurin represents a prime candidate. We hereby report that in cultured cerebellar granule cells the activation of the Ca2+/calcineurin pathway via either voltage- or ligand-operated Ca2+ channels regulates MALS-1 and MALS-2 expression at the transcriptional level. These proteins are integral parts of the post-synaptic density and are also involved in receptor trafficking. MALS regulation is not at the level of mRNA stability and does not require de novo protein synthesis, thereby suggesting a direct pathway. These data suggest that Ca2+ entry by means of calcineurin is capable of controlling the structure of the post-synaptic density by controlling the expression of key components at the transcriptional level.
UR - http://www.scopus.com/inward/record.url?scp=0037147236&partnerID=8YFLogxK
U2 - 10.1074/jbc.M207826200
DO - 10.1074/jbc.M207826200
M3 - Article
SN - 0021-9258
VL - 277
SP - 49585
EP - 49590
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -