TY - JOUR
T1 - THE EFFECT OF PREOPERATIVE LOCAL INTERLEUKIN-2 (IL-2) INJECTIONS IN PATIENTS WITH HEAD AND NECK SQUAMOUS-CELL CARCINOMA - AN IMMUNOLOGICAL STUDY
AU - CORTESINA, G
AU - DESTEFANI, A
AU - GALEAZZI, E
AU - BUSSI, M
AU - GIORDANO, C
AU - GP, CAVALLO
AU - JEMMA, C
AU - VAI, S
AU - FORNI, G
AU - VALENTE, Guido
N1 - Funding Information:
This work was supported by grant from Istituto Superiore di Sanith (ISS), Italy-USA Cancer Therapy and MPI 40 %.
PY - 1991
Y1 - 1991
N2 - Clinical, immunological, immunophenotypical, pathological and molecular biological studies were performed on tumor infiltrating lymphocytes (TIL) and lymph node lymphocytes (LN-ly) of 8 patients with squamous cell carcinoma of the oral cavity and oropharynx treated with 10 daily locoregional injections of low doses of IL-2 before surgery. No complications were seen during or after surgery. In 3 cases the LN-ly showed a moderate LAK activity, higher in the LN-ly omolateral to the tumor and near the IL-2 injection site; in 2 of these 3 patients a good LAK activity was induced after 6-day culture with IL-2. The LN-ly derived from nodes next to the tumor showed a decreased NK activity and proliferative ability both in basal conditions and after in vitro lymphokine challenging. LN-ly of 2 IL-2 treated patients showed high levels of mRNA encoding for IL-2-R, while it was absent in 2 untreated cases. Immunophenotypical studies on TIL showed statistically improved levels of CD25+ and LAK1+ cells in treated cases. Clusters of CD11c+ (macrophages) cells were seen close to the neoplastic sheets.
AB - Clinical, immunological, immunophenotypical, pathological and molecular biological studies were performed on tumor infiltrating lymphocytes (TIL) and lymph node lymphocytes (LN-ly) of 8 patients with squamous cell carcinoma of the oral cavity and oropharynx treated with 10 daily locoregional injections of low doses of IL-2 before surgery. No complications were seen during or after surgery. In 3 cases the LN-ly showed a moderate LAK activity, higher in the LN-ly omolateral to the tumor and near the IL-2 injection site; in 2 of these 3 patients a good LAK activity was induced after 6-day culture with IL-2. The LN-ly derived from nodes next to the tumor showed a decreased NK activity and proliferative ability both in basal conditions and after in vitro lymphokine challenging. LN-ly of 2 IL-2 treated patients showed high levels of mRNA encoding for IL-2-R, while it was absent in 2 untreated cases. Immunophenotypical studies on TIL showed statistically improved levels of CD25+ and LAK1+ cells in treated cases. Clusters of CD11c+ (macrophages) cells were seen close to the neoplastic sheets.
UR - https://iris.uniupo.it/handle/11579/5135
U2 - 10.3109/00016489109137414
DO - 10.3109/00016489109137414
M3 - Article
SN - 0001-6489
VL - 111
SP - 428
EP - 433
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - 2
ER -