The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments

Daniele Sblattero, Fiorella Florian, Elisabetta Azzoni, Trevin Zyla, Min Park, Valentina Baldas, Tarcisio Not, Alessandro Ventura, Andrew Bradbury, Roberto Marzari

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Celiac disease is an intestinal malabsorption characterized by an intolerance to cereal proteins accompanied by immunological responses to dietary gliadins and an autoantigen located in the endomysium. The latter has been identified as the enzyme tissue transglutaminase which belongs to a family of enzymes that catalyze protein cross-linking reactions and is constitutively expressed in many tissues as well as being activated during apoptosis. In a recent paper, we described the selection and characterization of anti-transglutaminase Igs from phage antibody libraries created from intestinal lymphocytes from celiac disease patients. In this work, using transglutaminase gene fragments, we identify a region of tissue transglutaminase recognized by these antibodies as being conformational and located in the core domain of the enzyme. This is identical to the region recognized by anti-transglutaminase Igs found in the serum of celiac disease patients.

Lingua originaleInglese
pagine (da-a)5175-5181
Numero di pagine7
RivistaEuropean Journal of Biochemistry
Volume269
Numero di pubblicazione21
DOI
Stato di pubblicazionePubblicato - 2002
Pubblicato esternamente

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