The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro Ravera; Graziana Bagni; Marco Mascini; James C. Dabrowiak; Domenico Osella

doi:10.1016/j.jinorgbio.2007.03.017

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro Ravera, Graziana Bagni, Marco Mascini, James C. Dabrowiak, Domenico Osella

Dipartimento di Scienze e Innovazione Tecnologica

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

Lingua originale	Inglese
pagine (da-a)	1023-1027
Numero di pagine	5
Rivista	Journal of Inorganic Biochemistry
Volume	101
Numero di pubblicazione	7
DOI	https://doi.org/10.1016/j.jinorgbio.2007.03.017
Stato di pubblicazione	Pubblicato - lug 2007

Accesso al documento

10.1016/j.jinorgbio.2007.03.017

Altri file e collegamenti

Link to publication in Scopus

Cita questo

@article{6545cad3da1846c78e0a7b815567fcba,

title = "The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor",

abstract = "We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.",

keywords = "Carbonate buffer, DNA-biosensor, Platinum drugs",

author = "Mauro Ravera and Graziana Bagni and Marco Mascini and Dabrowiak, {James C.} and Domenico Osella",

note = "Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).",

year = "2007",

month = jul,

doi = "10.1016/j.jinorgbio.2007.03.017",

language = "English",

volume = "101",

pages = "1023--1027",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

AU - Ravera, Mauro

AU - Bagni, Graziana

AU - Mascini, Marco

AU - Dabrowiak, James C.

AU - Osella, Domenico

N1 - Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).

PY - 2007/7

Y1 - 2007/7

N2 - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

AB - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

KW - Carbonate buffer

KW - DNA-biosensor

KW - Platinum drugs

UR - http://www.scopus.com/inward/record.url?scp=34250192567&partnerID=8YFLogxK

U2 - 10.1016/j.jinorgbio.2007.03.017

DO - 10.1016/j.jinorgbio.2007.03.017

M3 - Article

SN - 0162-0134

VL - 101

SP - 1023

EP - 1027

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 7

ER -

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Abstract

Accesso al documento

Altri file e collegamenti

Fingerprint

Cita questo