Temperature dependence of retention in reversed-phase ion-interaction chromatography

M. C. Gennaro; D. Giacosa; C. Abrigo; E. Marengo

doi:10.1093/chromsci/33.7.360

Temperature dependence of retention in reversed-phase ion-interaction chromatography

M. C. Gennaro, D. Giacosa, C. Abrigo, E. Marengo

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The dependence of retention on temperature in reversed-phase ion-interaction chromatography is investigated. 5mM octylammonium o-phosphate in aqueous solution is used as the interaction reagent, and retention of analytes characterized by different chemical properties is considered in the temperature range of 25-55°C. Variation of the thermodynamic parameters of enthalpy and entropy for the transfer process of the solute from the mobile to the stationary phase are evaluated. The results indicate that the retention process proceeds in the temperature range investigated through only a single mechanism and that similar retention mechanisms characterize all the analytes studied, independent of their chemical structure.

Lingua originale	Inglese
pagine (da-a)	360-364
Numero di pagine	5
Rivista	Journal of Chromatographic Science
Volume	33
Numero di pubblicazione	7
DOI	https://doi.org/10.1093/chromsci/33.7.360
Stato di pubblicazione	Pubblicato - lug 1995
Pubblicato esternamente	Sì

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title = "Temperature dependence of retention in reversed-phase ion-interaction chromatography",

abstract = "The dependence of retention on temperature in reversed-phase ion-interaction chromatography is investigated. 5mM octylammonium o-phosphate in aqueous solution is used as the interaction reagent, and retention of analytes characterized by different chemical properties is considered in the temperature range of 25-55°C. Variation of the thermodynamic parameters of enthalpy and entropy for the transfer process of the solute from the mobile to the stationary phase are evaluated. The results indicate that the retention process proceeds in the temperature range investigated through only a single mechanism and that similar retention mechanisms characterize all the analytes studied, independent of their chemical structure.",

author = "Gennaro, {M. C.} and D. Giacosa and C. Abrigo and E. Marengo",

note = "Funding Information: This work was carried out with the financial support of the Italian Consiglio Nazionale delle Ricerche (CNR) and the Ministero dell'Universita e della Ricerca Scientifica e Tecno-logica (MURST).",

year = "1995",

month = jul,

doi = "10.1093/chromsci/33.7.360",

language = "English",

volume = "33",

pages = "360--364",

journal = "Journal of Chromatographic Science",

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publisher = "Oxford University Press",

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TY - JOUR

T1 - Temperature dependence of retention in reversed-phase ion-interaction chromatography

AU - Gennaro, M. C.

AU - Giacosa, D.

AU - Abrigo, C.

AU - Marengo, E.

N1 - Funding Information: This work was carried out with the financial support of the Italian Consiglio Nazionale delle Ricerche (CNR) and the Ministero dell'Universita e della Ricerca Scientifica e Tecno-logica (MURST).

PY - 1995/7

Y1 - 1995/7

N2 - The dependence of retention on temperature in reversed-phase ion-interaction chromatography is investigated. 5mM octylammonium o-phosphate in aqueous solution is used as the interaction reagent, and retention of analytes characterized by different chemical properties is considered in the temperature range of 25-55°C. Variation of the thermodynamic parameters of enthalpy and entropy for the transfer process of the solute from the mobile to the stationary phase are evaluated. The results indicate that the retention process proceeds in the temperature range investigated through only a single mechanism and that similar retention mechanisms characterize all the analytes studied, independent of their chemical structure.

AB - The dependence of retention on temperature in reversed-phase ion-interaction chromatography is investigated. 5mM octylammonium o-phosphate in aqueous solution is used as the interaction reagent, and retention of analytes characterized by different chemical properties is considered in the temperature range of 25-55°C. Variation of the thermodynamic parameters of enthalpy and entropy for the transfer process of the solute from the mobile to the stationary phase are evaluated. The results indicate that the retention process proceeds in the temperature range investigated through only a single mechanism and that similar retention mechanisms characterize all the analytes studied, independent of their chemical structure.

UR - http://www.scopus.com/inward/record.url?scp=84959808555&partnerID=8YFLogxK

U2 - 10.1093/chromsci/33.7.360

DO - 10.1093/chromsci/33.7.360

M3 - Article

SN - 0021-9665

VL - 33

SP - 360

EP - 364

JO - Journal of Chromatographic Science

JF - Journal of Chromatographic Science

IS - 7

ER -

Temperature dependence of retention in reversed-phase ion-interaction chromatography

Abstract

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