TY - JOUR
T1 - Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots
AU - Repetto, Ombretta
AU - Bestel-Corre, Gwénäelle
AU - Dumas-Gaudot, Eliane
AU - Berta, Graziella
AU - Gianinazzi-Pearson, Vivienne
AU - Gianinazzi, Silvio
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. The effect of Cd (100 mg Cd kg-1 substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses. Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography-tandem mass spectrometry, newly induced and upregulated or downregulated by Cd. The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants.
AB - Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. The effect of Cd (100 mg Cd kg-1 substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses. Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography-tandem mass spectrometry, newly induced and upregulated or downregulated by Cd. The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants.
KW - Cadmium stress
KW - Glomus mosseae
KW - Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
KW - One- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses
KW - Pisum sativum
KW - Plant biomass
KW - Protein profiles
UR - http://www.scopus.com/inward/record.url?scp=0037341977&partnerID=8YFLogxK
U2 - 10.1046/j.1469-8137.2003.00682.x
DO - 10.1046/j.1469-8137.2003.00682.x
M3 - Article
SN - 0028-646X
VL - 157
SP - 555
EP - 567
JO - New Phytologist
JF - New Phytologist
IS - 3
ER -