Abstract
A fluorescent derivative of sulfatide, labeled with a dansyl probe at position 6 of galactose, was prepared through a glycosylation reaction between a 6-O-functionalized galactose donor and 3-O-benzoyl-azidosphingosine. The sphingoid acceptor was obtained in high yield through exploitation of a highly stereoselective nucleophilic addition of pentadecyne to 2,3-O-cyclohexylidene-D- glyceraldehyde. The fluorescent sulfatide binds to soluble recombinant human CD1a and is a valuable tool for the study of CD1 antigen binding properties.
Lingua originale | Inglese |
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pagine (da-a) | 4755-4761 |
Numero di pagine | 7 |
Rivista | European Journal of Organic Chemistry |
Numero di pubblicazione | 23 |
DOI | |
Stato di pubblicazione | Pubblicato - 26 nov 2004 |