TY - JOUR
T1 - Synthesis and characterization of silica-coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells
AU - Multari, Cristina
AU - Miola, Marta
AU - Ferraris, Sara
AU - Movia, Dania
AU - Žužek Rožman, Kristina
AU - Kostevšek, Nina
AU - Follenzi, Antonia
AU - Verné, Enrica
AU - Prina-Mello, Adriele
N1 - Publisher Copyright:
© 2018 The American Ceramic Society
PY - 2018/7/1
Y1 - 2018/7/1
N2 - The aim of the study is to investigate the relationship between the physico-chemical properties of superparamagnetic iron oxide nanoparticles (SPIONs) and their cytotoxicity profile in light of their potential biomedical application as nanocarriers for pancreatic cancer treatment. Two types of SPIONs were tested: magnetite nanoparticles (Fe3O4 NPs) and silica-coated magnetite nanoparticles (SiO2-Fe3O4 NPs). The physico-chemical properties of the 2 SPIONs were characterized by means of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectrometry (EDS), and Selected Area Electron Diffraction (SAED). Their magnetic properties were quantified as magnetization saturation (Ms) and Remanence. The colloidal stability was investigated by Isoelectric Point Measurements and sedimentation tests. Finally, in vitro characterizations were performed to quantify the half maximal lethal concentration (LC50), by means of High Content Screening Analysis (HCSA), Flow cytometry (FC), and Laser Scanning Confocal Microscopy (LSCM). The obtained NPs present a spherical shape and a dimension between 10 and 20 nm, a superparamagnetic behavior and surface charge in agreement with their surface chemistry. The in vitro tests demonstrate that both NPs induce similar levels of cytotoxicity in a PANC-1 cell model and were internalized, with SiO2-Fe3O4 NPs associated to a slightly higher cellular internalization, probably due to their higher dispersability.
AB - The aim of the study is to investigate the relationship between the physico-chemical properties of superparamagnetic iron oxide nanoparticles (SPIONs) and their cytotoxicity profile in light of their potential biomedical application as nanocarriers for pancreatic cancer treatment. Two types of SPIONs were tested: magnetite nanoparticles (Fe3O4 NPs) and silica-coated magnetite nanoparticles (SiO2-Fe3O4 NPs). The physico-chemical properties of the 2 SPIONs were characterized by means of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectrometry (EDS), and Selected Area Electron Diffraction (SAED). Their magnetic properties were quantified as magnetization saturation (Ms) and Remanence. The colloidal stability was investigated by Isoelectric Point Measurements and sedimentation tests. Finally, in vitro characterizations were performed to quantify the half maximal lethal concentration (LC50), by means of High Content Screening Analysis (HCSA), Flow cytometry (FC), and Laser Scanning Confocal Microscopy (LSCM). The obtained NPs present a spherical shape and a dimension between 10 and 20 nm, a superparamagnetic behavior and surface charge in agreement with their surface chemistry. The in vitro tests demonstrate that both NPs induce similar levels of cytotoxicity in a PANC-1 cell model and were internalized, with SiO2-Fe3O4 NPs associated to a slightly higher cellular internalization, probably due to their higher dispersability.
KW - magnetic materials/properties
KW - nanomaterials
KW - nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85044375495&partnerID=8YFLogxK
U2 - 10.1111/ijac.12897
DO - 10.1111/ijac.12897
M3 - Article
SN - 1546-542X
VL - 15
SP - 947
EP - 960
JO - International Journal of Applied Ceramic Technology
JF - International Journal of Applied Ceramic Technology
IS - 4
ER -
