Synthesis and Characterization of a Novel DTPA-like Gadolinium(III) Complex: A Potential Reagent for the Determination of Glycated Proteins by Water Proton NMR Relaxation Measurements

Silvio Aime, Mauro Botta, Walter Dastrú, Mauro Fasano, Maurizio Panero, Aldo Arnelli

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

The synthesis of a novel DTPA-like ligand 2 containing two boronic acid phenylamide substituents and its Gd(III) complex 3 is reported. The measurement of the proton relaxation rate over the proton Larmor frequency range from 0.01 to 50 MHz of an aqueous solution of this paramagnetic complex suggests that it contains only one water molecule in its inner coordination sphere, as was previously found for the parent DTPA complex. Competition trials in presence of variable amounts of [Gd-EDTA] afford a Kf value of 2.0 × 1016. Since the boronic functionalities form stable linkages with sin-diol moieties, the interaction of complex 3 with glycated albumin leads to the formation of a ternary albumin-3 complex. The large size of this adduct is responsible for a lengthening of the molecular reorientational time τR, which in turn results in an increase of water proton relaxation rate (R1p). The observed R1p enhancement then provides a direct evaluation of the extent of glycation of the albumin specimen.

Lingua originaleInglese
pagine (da-a)2068-2071
Numero di pagine4
RivistaInorganic Chemistry
Volume32
Numero di pubblicazione10
DOI
Stato di pubblicazionePubblicato - 1993
Pubblicato esternamente

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