Suitability of different C18 silica-based stationary phases for the transferability of an ion-interaction HPLC method

M. C. Gennaro, S. Angelino, V. Gianotti

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

A critical chromatographic separation of a mixture containing inorganic anions (nitrate and nitrite) and organic cations (the three isomeric forms of phenylendiamine) was chosen as the System Suitability Test (SST) to compare the performance, under Ion-Interaction condition, of five different C18 columns characterised by the same specifics. The raggedness (i.e. the capacity to yield good results also when there are variations in the 'experimental circumstances') of the Ion Interaction Reagent (IIR) HPLC method previously developed and optimised was evaluated with robustness tests for each stationary phase considered. Variations of ±10 % to the nominal values of the chromatographic conditions were imposed and the effects on the chromatographic response were evaluated. For the different stationary phases the results showed performances and behaviours unexpectedly different, concerning not only the resolution among the components of the mixture but also their elution sequence order. The studies identified the pH value of the mobile phase as the most critical parameter of the method, which must he very strictly controlled. For one of the stationary phases considered a pH variation of only 5 % with respect to the nominal value not only gave unsatisfactory resolutions, but also a different elution order of the analytes. Another of the tested columns was proved not suitable to work under these IIR conditions.

Lingua originaleInglese
pagine (da-a)742-748
Numero di pagine7
RivistaAnalusis
Volume27
Numero di pubblicazione8
DOI
Stato di pubblicazionePubblicato - ott 1999

Fingerprint

Entra nei temi di ricerca di 'Suitability of different C18 silica-based stationary phases for the transferability of an ion-interaction HPLC method'. Insieme formano una fingerprint unica.

Cita questo