TY - JOUR
T1 - Studies on fatty liver with isolated hepatocytes. III. Cumene hydroperoxide-induced change of several cell functions
AU - Chiarpotto, Elena
AU - Poli, Giuseppe
AU - Albano, Emanuele
AU - Gravela, Enrico
AU - Dianzani, Mario U.
N1 - Funding Information:
The authors dedicate this publication to Professor Erwin Schauenstein, Head of the Institute of Biochemistry at the Karl-Franzens University in Graz, Austria, on the occasion of his 65th birthday. These studies have been sponsored by the National Foundation for Cancer Research, Bethesda. Maryland, and by the Consiglio Nazionale delle Ricerche, Rome, Italy.
PY - 1984/10
Y1 - 1984/10
N2 - Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.
AB - Isolated rat hepatocytes have been treated with cumene hydroperoxide at concentrations not inducing irreversible cell damage. Under these experimental conditions cells show an enhanced lipid peroxidation, a decrease of glucose-6-phosphatase activity and of cytochrome P-450 content, and a stimulation of aminopyrene demethylation. Furthermore, the hepatocyte incorporation of amino acids is slightly but significantly reduced by the tested compound. Finally, because of the inhibitory effect of cumene hydroperoxide on cell lipoprotein but not on protein secretion, a mechanism of damage acting at the level of the assembly and maturation of lipoprotein micelles is postulated.
UR - http://www.scopus.com/inward/record.url?scp=0021711451&partnerID=8YFLogxK
U2 - 10.1016/0014-4800(84)90035-2
DO - 10.1016/0014-4800(84)90035-2
M3 - Article
SN - 0014-4800
VL - 41
SP - 191
EP - 201
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 2
ER -