Structure of human NMN adenylyltransferase: A key nuclear enzyme for NAD homeostasis

Nicotinamide mononucleotide adenylyltransferase (NMNAT), a member of the nucleotidyltransferase α/β-phosphodiesterases superfamily, catalyzes a universal step (NMN + ATP = NAD + PP_i) in NAD biosynthesis. Localized within the nucleus, the activity of the human enzyme is greatly altered in tumor cells, rendering it a promising target for cancer chemotherapy. By using a combination of single isomorphous replacement and density modification techniques, the human NMNAT structure was solved by x-ray crystallography to a 2.5-Å resolution, revealing a hexamer that is composed of α/β-topology subunits. The active site topology of the enzyme, analyzed through homology modeling and structural comparison with other NMNATs, yielded convincing evidence for a substrate-induced conformational change. We also observed remarkable structural conservation in the ATP-recognition motifs GXXXPX-(T/H)XXH and SXTXXR, which we take to be the universal signature for NMNATs. Structural comparison of human and prokaryotic NMNATs may also lead to the rational design of highly selective antimicrobial drugs.