TY - JOUR
T1 - STR typing of fixed human tissue
T2 - Formalin vs. an alcohol-based method
AU - Gino, Sarah
AU - Varacalli, Simona
AU - Robino, Carlo
AU - Torre, Carlo
PY - 2004/4/1
Y1 - 2004/4/1
N2 - In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad®). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.
AB - In this study, we evaluated the effect on DNA typing of storage of human tissue in formalin versus an alcohol-based method (Complucad®). Samples of tissue were collected at different time from fixation. DNA was isolated and, after quantitation, was amplified by quadruplex PCR including four STR loci (LPL, F13B, FESFPS and F13A01). For alcohol-fixed tissue, quantitation showed that DNA yield remained unchanged regardless of storage time and complete profiles were obtained. Instead, the DNA amount recovered from those fixed with formalin was lower and DNA looked very degraded. Actually, for these samples, we observed a lack of amplification of >200 bp loci.
KW - Forensic casework
KW - Paternity testing
KW - STR
KW - Tissue fixation
UR - https://www.scopus.com/pages/publications/33344459817
U2 - 10.1016/S0531-5131(03)01660-1
DO - 10.1016/S0531-5131(03)01660-1
M3 - Article
AN - SCOPUS:33344459817
SN - 0531-5131
VL - 1261
SP - 611
EP - 612
JO - International Congress Series
JF - International Congress Series
IS - C
ER -