TY - JOUR
T1 - Stem cell-extracellular vesicles as drug delivery systems
T2 - New frontiers for silk/curcumin nanoparticles
AU - Perteghella, Sara
AU - Crivelli, Barbara
AU - Catenacci, Laura
AU - Sorrenti, Milena
AU - Bruni, Giovanna
AU - Necchi, Vittorio
AU - Vigani, Barbara
AU - Sorlini, Marzio
AU - Torre, Maria Luisa
AU - Chlapanidas, Theodora
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/3/30
Y1 - 2017/3/30
N2 - The aim of this work was to develop a novel carrier-in-carrier system based on stem cell-extracellular vesicles loaded of silk/curcumin nanoparticles by endogenous technique. Silk nanoparticles were produced by desolvation method and curcumin has been selected as drug model because of its limited water solubility and poor bioavailability. Nanoparticles were stable, with spherical geometry, 100 nm in average diameter and the drug content reached about 30%. Cellular uptake studies, performed on mesenchymal stem cells (MSCs), showed the accumulation of nanoparticles in the cytosol around the nuclear membrane, without cytotoxic effects. Finally, MSCs were able to release extracellular vesicles entrapping silk/curcumin nanoparticles. This combined biological-technological approach represents a novel class of nanosystems, combining beneficial effects of both regenerative cell therapies and pharmaceutical nanomedicine, avoiding the use of viable replicating stem cells.
AB - The aim of this work was to develop a novel carrier-in-carrier system based on stem cell-extracellular vesicles loaded of silk/curcumin nanoparticles by endogenous technique. Silk nanoparticles were produced by desolvation method and curcumin has been selected as drug model because of its limited water solubility and poor bioavailability. Nanoparticles were stable, with spherical geometry, 100 nm in average diameter and the drug content reached about 30%. Cellular uptake studies, performed on mesenchymal stem cells (MSCs), showed the accumulation of nanoparticles in the cytosol around the nuclear membrane, without cytotoxic effects. Finally, MSCs were able to release extracellular vesicles entrapping silk/curcumin nanoparticles. This combined biological-technological approach represents a novel class of nanosystems, combining beneficial effects of both regenerative cell therapies and pharmaceutical nanomedicine, avoiding the use of viable replicating stem cells.
KW - Curcumin
KW - Drug delivery systems
KW - Extracellular vesicles
KW - Mesenchymal stem cells
KW - Nanoparticles
KW - Silk fibroin
UR - https://www.scopus.com/pages/publications/85011700323
U2 - 10.1016/j.ijpharm.2017.02.005
DO - 10.1016/j.ijpharm.2017.02.005
M3 - Article
SN - 0378-5173
VL - 520
SP - 86
EP - 97
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -