TY - JOUR
T1 - Somatostatin in lungfish kidney
T2 - An immunohistochemical, autoradiographical and in situ hybridisation study
AU - Masini, Maria Angela
AU - Sturla, Maddalena
AU - Uva, Bianca Maria
N1 - Funding Information:
This work was supported by a grant from the University of Genova.
PY - 1998/1
Y1 - 1998/1
N2 - The localisation of somatostatin-14 (SST-14) was examined immunohistochemically using the antibody Ab-SST-14 in the kidney of the African lungfish Protopterus annectens. Immunoreactive cells were present in the proximal tubules. In situ hybridisation, using an oligonucleotide probe complementary to mRNA for SST-14 and labeled at the 3'-end with α-35S, showed SST-14 mRNA distributed in cells with the same localisation as seen for SST-14 immunoreactive cells. Binding sites for SST-14 were identified with autoradiography using 125I SST-14. Binding sites were concentrated on cells of the proximal tubules. It is suggested that SST-14 may be synthesised in the lungfish mesonephros.
AB - The localisation of somatostatin-14 (SST-14) was examined immunohistochemically using the antibody Ab-SST-14 in the kidney of the African lungfish Protopterus annectens. Immunoreactive cells were present in the proximal tubules. In situ hybridisation, using an oligonucleotide probe complementary to mRNA for SST-14 and labeled at the 3'-end with α-35S, showed SST-14 mRNA distributed in cells with the same localisation as seen for SST-14 immunoreactive cells. Binding sites for SST-14 were identified with autoradiography using 125I SST-14. Binding sites were concentrated on cells of the proximal tubules. It is suggested that SST-14 may be synthesised in the lungfish mesonephros.
UR - http://www.scopus.com/inward/record.url?scp=0031912486&partnerID=8YFLogxK
U2 - 10.1006/gcen.1997.6991
DO - 10.1006/gcen.1997.6991
M3 - Article
SN - 0016-6480
VL - 109
SP - 1
EP - 7
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -