TY - JOUR
T1 - Skeletal muscle differentiation potential of human adult bone marrow cells
AU - Bossolasco, Patrizia
AU - Corti, Stefania
AU - Strazzer, Sandra
AU - Borsotti, Chiara
AU - Del Bo, Roberto
AU - Fortunato, Francesco
AU - Salani, Sabrina
AU - Quirici, Nadia
AU - Bertolini, Francesco
AU - Gobbi, Alberto
AU - Deliliers, Giorgio Lambertenghi
AU - Comi, Giacomo Pietro
AU - Soligo, Davide
N1 - Funding Information:
This research was funded by the Ministero della Salute, Italy (R.F. Stem Cell 2001 to D.S. and G.P.C.).
PY - 2004/4/15
Y1 - 2004/4/15
N2 - Murine bone marrow (BM) cells have been shown to undergo myogenic differentiation and participate in muscle repair in different muscle regeneration models. In the present paper, we report on a subset of cells (CD45+/desmin+) with myogenic potential being present at very low frequencies in human adult BM. By a simple culture method, we were able to obtain in vitro multinucleated myotubes in up to 20% of the cultures. Myotubes were generated using both BM flushed from rib fragments obtained during thoracotomy and BM derived from iliac crest aspirates. Cells of the different adherent and non-adherent fractions expressed numerous muscle specific markers by immunocytochemistry, real-time RT-PCR, flow cytometry, and Western blot analyses. Moreover, direct injection of whole BM into the right tibialis anterior muscle of immunodeficient mice (NOD/RAG) that had previously been treated with cardiotoxin to induce muscle degeneration, showed a variable but significant level of human cell engraftment (from 0.06 to 0.26% Dys+/FISH+ fibers). These data suggest that cells with skeletal muscle differentiation potential are present in adult human BM can differentiate in vitro and give rise to myogenic cells in vivo in immunodeficient mice after muscle damage. Further improvements might allow new approaches to cell-mediated therapies for muscular diseases.
AB - Murine bone marrow (BM) cells have been shown to undergo myogenic differentiation and participate in muscle repair in different muscle regeneration models. In the present paper, we report on a subset of cells (CD45+/desmin+) with myogenic potential being present at very low frequencies in human adult BM. By a simple culture method, we were able to obtain in vitro multinucleated myotubes in up to 20% of the cultures. Myotubes were generated using both BM flushed from rib fragments obtained during thoracotomy and BM derived from iliac crest aspirates. Cells of the different adherent and non-adherent fractions expressed numerous muscle specific markers by immunocytochemistry, real-time RT-PCR, flow cytometry, and Western blot analyses. Moreover, direct injection of whole BM into the right tibialis anterior muscle of immunodeficient mice (NOD/RAG) that had previously been treated with cardiotoxin to induce muscle degeneration, showed a variable but significant level of human cell engraftment (from 0.06 to 0.26% Dys+/FISH+ fibers). These data suggest that cells with skeletal muscle differentiation potential are present in adult human BM can differentiate in vitro and give rise to myogenic cells in vivo in immunodeficient mice after muscle damage. Further improvements might allow new approaches to cell-mediated therapies for muscular diseases.
KW - Bone marrow
KW - Hematopoietic stem cells
KW - Muscle cells
KW - Myotubes
KW - Plasticity
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=12144290852&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2003.12.015
DO - 10.1016/j.yexcr.2003.12.015
M3 - Article
SN - 0014-4827
VL - 295
SP - 66
EP - 78
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -