Simulated microgravity induces nuclear translocation of Bax and BCL-2 in glial cultured C6 cells

Tommaso Bonfiglio, Federico Biggi, Anna Maria Bassi, Sara Ferrando, Lorenzo Gallus, Fabrizio Loiacono, Silvia Ravera, Marino Rottigni, Sonia Scarfì, Felice Strollo, Stefania Vernazza, Maurizio Sabbatini, Maria A. Masini

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Alterations in the control of apoptotic processes were observed in cells during space flight or under simulated microgravity, the latter obtained with the 3D-Random Positioning Machine (3D-RPM). Usually the proteins Bax and Bcl-2, act as pro- or anti-apoptotic regulators. Here we investigated the effects of simulated microgravity obtained by the 3D-RPM on cell viability, localization and expression of Bax and Bcl-2 in cultures of glial cancerous cells. We observed for the first time a transient cytoplasmic/nuclear translocation of Bax and Bcl-2 triggered by changing gravity vector. Bax translocates into the nucleus after 1 h, is present simultaneously in the cytoplasm after 6 h and comes back to the cytoplasm after 24 h. Bcl-2 translocate into the nucleus only after 6 h and comes back to the cytoplasm after 24 h. Physiological meaning, on the regulation of apoptotic event and possible applicative outcomes of such finding are discussed.

Lingua originaleInglese
Numero di articoloe01798
RivistaHeliyon
Volume5
Numero di pubblicazione6
DOI
Stato di pubblicazionePubblicato - giu 2019

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