Shc signaling in differentiating neural progenitor cells

Luciano Conti; Simonetta Sipione; Lorenzo Magrassi; Luca Bonfanti; Dorotea Rigamonti; Valentina Pettirossi; Marc Peschanski; Bassam Haddad; Pier Giuseppe Pelicci; Gabriele Milanesi; Giuliana Pelicci; Elena Cattaneo

doi:10.1038/88395

Shc signaling in differentiating neural progenitor cells

Luciano Conti, Simonetta Sipione, Lorenzo Magrassi, Luca Bonfanti, Dorotea Rigamonti, Valentina Pettirossi, Marc Peschanski, Bassam Haddad, Pier Giuseppe Pelicci, Gabriele Milanesi, Giuliana Pelicci, Elena Cattaneo

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Previously we found that the availability of ShcA adapter is maximal in neural stem cells but that it is absent in mature neurons. Here we report that ShcC, unlike ShcA, is not present in neural stem/progenitor cells, but is expressed after cessation of their division and becomes selectively enriched in mature neurons. Analyses of its activity in differentiating neural stem/progenitor cells revealed that ShcC positively affects their viability and neuronal maturation via recruitment of the PI3K-Akt-Bad pathway and persistent activation of the MAPK pathway. We suggest that the switch from ShcA to ShcC modifies the responsiveness of neural stem/progenitor cells to extracellular stimuli, generating proliferation (with ShcA) or survival/differentiation (with ShcC).

Lingua originale	Inglese
pagine (da-a)	579-586
Numero di pagine	8
Rivista	Nature Neuroscience
Volume	4
Numero di pubblicazione	6
DOI	https://doi.org/10.1038/88395
Stato di pubblicazione	Pubblicato - 2001
Pubblicato esternamente	Sì

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title = "Shc signaling in differentiating neural progenitor cells",

abstract = "Previously we found that the availability of ShcA adapter is maximal in neural stem cells but that it is absent in mature neurons. Here we report that ShcC, unlike ShcA, is not present in neural stem/progenitor cells, but is expressed after cessation of their division and becomes selectively enriched in mature neurons. Analyses of its activity in differentiating neural stem/progenitor cells revealed that ShcC positively affects their viability and neuronal maturation via recruitment of the PI3K-Akt-Bad pathway and persistent activation of the MAPK pathway. We suggest that the switch from ShcA to ShcC modifies the responsiveness of neural stem/progenitor cells to extracellular stimuli, generating proliferation (with ShcA) or survival/differentiation (with ShcC).",

author = "Luciano Conti and Simonetta Sipione and Lorenzo Magrassi and Luca Bonfanti and Dorotea Rigamonti and Valentina Pettirossi and Marc Peschanski and Bassam Haddad and Pelicci, {Pier Giuseppe} and Gabriele Milanesi and Giuliana Pelicci and Elena Cattaneo",

note = "Funding Information: This work was supported by Associazione Italiana Ricerca Cancro and Ministry of University and Technological Research (Italy #9905261712) to E.C., by Telethon (Italy, E1025) to L.C., and Telethon (Italy, A128) and C.N.R. (P.S. Basi Biologiche Malattie Neurodegenerative) to L.M. We thank R. DeBona and M. De Simone for their help with the cell cultures and immunoreactions, and F. Benvenuto for help with the FACS analysis. We also thank P. Delli Santi for her technical support and L. Chiappino for her photographic expertise.",

year = "2001",

doi = "10.1038/88395",

language = "English",

volume = "4",

pages = "579--586",

journal = "Nature Neuroscience",

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T1 - Shc signaling in differentiating neural progenitor cells

AU - Conti, Luciano

AU - Sipione, Simonetta

AU - Magrassi, Lorenzo

AU - Bonfanti, Luca

AU - Rigamonti, Dorotea

AU - Pettirossi, Valentina

AU - Peschanski, Marc

AU - Haddad, Bassam

AU - Pelicci, Pier Giuseppe

AU - Milanesi, Gabriele

AU - Pelicci, Giuliana

AU - Cattaneo, Elena

N1 - Funding Information: This work was supported by Associazione Italiana Ricerca Cancro and Ministry of University and Technological Research (Italy #9905261712) to E.C., by Telethon (Italy, E1025) to L.C., and Telethon (Italy, A128) and C.N.R. (P.S. Basi Biologiche Malattie Neurodegenerative) to L.M. We thank R. DeBona and M. De Simone for their help with the cell cultures and immunoreactions, and F. Benvenuto for help with the FACS analysis. We also thank P. Delli Santi for her technical support and L. Chiappino for her photographic expertise.

PY - 2001

Y1 - 2001

N2 - Previously we found that the availability of ShcA adapter is maximal in neural stem cells but that it is absent in mature neurons. Here we report that ShcC, unlike ShcA, is not present in neural stem/progenitor cells, but is expressed after cessation of their division and becomes selectively enriched in mature neurons. Analyses of its activity in differentiating neural stem/progenitor cells revealed that ShcC positively affects their viability and neuronal maturation via recruitment of the PI3K-Akt-Bad pathway and persistent activation of the MAPK pathway. We suggest that the switch from ShcA to ShcC modifies the responsiveness of neural stem/progenitor cells to extracellular stimuli, generating proliferation (with ShcA) or survival/differentiation (with ShcC).

AB - Previously we found that the availability of ShcA adapter is maximal in neural stem cells but that it is absent in mature neurons. Here we report that ShcC, unlike ShcA, is not present in neural stem/progenitor cells, but is expressed after cessation of their division and becomes selectively enriched in mature neurons. Analyses of its activity in differentiating neural stem/progenitor cells revealed that ShcC positively affects their viability and neuronal maturation via recruitment of the PI3K-Akt-Bad pathway and persistent activation of the MAPK pathway. We suggest that the switch from ShcA to ShcC modifies the responsiveness of neural stem/progenitor cells to extracellular stimuli, generating proliferation (with ShcA) or survival/differentiation (with ShcC).

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JF - Nature Neuroscience

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ER -

Shc signaling in differentiating neural progenitor cells

Abstract

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