TY - JOUR
T1 - Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble fas ligand expression
T2 - An immunoenzymatic study from the Italian group of immunopathology
AU - Quaglino, Pietro
AU - Caproni, Marzia
AU - Antiga, Emiliano
AU - Del Bianco, Elena
AU - Osella-Abate, Simona
AU - Savoia, Paola
AU - Frezzolini, Alessandra
AU - Schena, Donatella
AU - Marzano, Angelo
AU - Volpi, Walter
AU - De Simone, Clara
AU - Parodi, Aurora
AU - Fabbri, Paolo
AU - Bernengo, Maria Grazia
PY - 2007/4
Y1 - 2007/4
N2 - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.
AB - Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN). Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL). Materials and Methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD). Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194). Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.
KW - Erythema multiforme
KW - Stevens-Johnson syndrome/toxic epidermal necrolysis
KW - Thymus- and activation-regulated chemokine
UR - http://www.scopus.com/inward/record.url?scp=34247548710&partnerID=8YFLogxK
U2 - 10.1159/000100880
DO - 10.1159/000100880
M3 - Article
SN - 1018-8665
VL - 214
SP - 296
EP - 304
JO - Dermatology
JF - Dermatology
IS - 4
ER -