TY - JOUR
T1 - Scratch wound closure of C2C12 mouse myoblasts is enhanced by human platelet lysate
AU - Ranzato, Elia
AU - Balbo, Valeria
AU - Boccafoschi, Francesca
AU - Mazzucco, Laura
AU - Burlando, Bruno
N1 - Funding Information:
This work was supported by grants from the University of Piemonte Orientale “Amedeo Avogadro”. ER and VB are recipients of Research Fellowships from the University of Piemonte Orientale and the Fondazione Cassa di Risparmio di Torino, respectively.
PY - 2009/9
Y1 - 2009/9
N2 - The effect of a platelet lysate (PL) on muscle wound healing, based on in vitro scratch wound of C2C12 mouse myoblasts, has been investigated. Cell viability assays show that PL induced an increase in cell proliferation at concentrations of 1-20%, but was slightly cytotoxic at 100%. PL promoted wound closure after scratch wounding of cell monolayers. The p38 inhibitor SB203580 and the PI3K inhibitor, wortmannin, decreased the PL effect, whereas the ERK inhibitor, PD98059, did not. Transwell migration of cells was also increased by PL, and although SB203580 abrogated this effect, wortmannin reduced it, whereas PD98059 was ineffective. Western blot analyses of scratch wounded cells showed activation of AKT and p38, while in the presence of PL there was a faster and sustained activation of AKT and p38 (up to 6 h), and a transient activation of ERK1/2. Taken together, the data show that PL promotes C2C12 wound healing by enhancing cell proliferation and motility.
AB - The effect of a platelet lysate (PL) on muscle wound healing, based on in vitro scratch wound of C2C12 mouse myoblasts, has been investigated. Cell viability assays show that PL induced an increase in cell proliferation at concentrations of 1-20%, but was slightly cytotoxic at 100%. PL promoted wound closure after scratch wounding of cell monolayers. The p38 inhibitor SB203580 and the PI3K inhibitor, wortmannin, decreased the PL effect, whereas the ERK inhibitor, PD98059, did not. Transwell migration of cells was also increased by PL, and although SB203580 abrogated this effect, wortmannin reduced it, whereas PD98059 was ineffective. Western blot analyses of scratch wounded cells showed activation of AKT and p38, while in the presence of PL there was a faster and sustained activation of AKT and p38 (up to 6 h), and a transient activation of ERK1/2. Taken together, the data show that PL promotes C2C12 wound healing by enhancing cell proliferation and motility.
KW - Akt
KW - MAP kinases
KW - PI3 kinase
KW - Scratch wound assay
KW - Transwell migration
UR - http://www.scopus.com/inward/record.url?scp=68949220080&partnerID=8YFLogxK
U2 - 10.1016/j.cellbi.2009.06.017
DO - 10.1016/j.cellbi.2009.06.017
M3 - Article
SN - 1065-6995
VL - 33
SP - 911
EP - 917
JO - Cell Biology International
JF - Cell Biology International
IS - 9
ER -