Role of the kinase activation loop on protein kinase C θ activity and intracellular localisation

Bianca Sparatore; Mario Passalacqua; Marco Pedrazzi; Sabina Ledda; Mauro Patrone; Deborah Gaggero; Sandro Pontremoli; Edon Melloni

doi:10.1016/S0014-5793(03)01073-1

Role of the kinase activation loop on protein kinase C θ activity and intracellular localisation

Bianca Sparatore, Mario Passalacqua, Marco Pedrazzi, Sabina Ledda, Mauro Patrone, Deborah Gaggero, Sandro Pontremoli, Edon Melloni

Dipartimento di Scienze e Innovazione Tecnologica

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Multiple protein kinase C (PKC) θ species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCθs localised in the detergent-soluble cell fraction have an M_r of 76 kDa (θ-76) and contain Thr⁵³⁸ or pThr⁵³⁸ in the kinase activation loop. In contrast, PKCθs localised in the Golgi complex have an M _r of 85 kDa (θ-85) and, although unphosphorylated at Thr ⁵³⁸, are catalytically active. Strikingly, only θ-76 species which are unphosphorylated at Thr⁵³⁸ can undergo autocatalytic conversion to θ-85. Moreover, a Thr⁵³⁸→Ala PKCθ mutant is constitutively localised in the Golgi complex, confirming that changes in the phosphorylation state of this residue play a pivotal role in the overall control of catalytic properties and localisation of this kinase.

Lingua originale	Inglese
pagine (da-a)	35-40
Numero di pagine	6
Rivista	FEBS Letters
Volume	554
Numero di pubblicazione	1-2
DOI	https://doi.org/10.1016/S0014-5793(03)01073-1
Stato di pubblicazione	Pubblicato - 6 nov 2003

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10.1016/S0014-5793(03)01073-1

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@article{35c135619d8a4ceea610a1b2c1625368,

title = "Role of the kinase activation loop on protein kinase C θ activity and intracellular localisation",

abstract = "Multiple protein kinase C (PKC) θ species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCθs localised in the detergent-soluble cell fraction have an Mr of 76 kDa (θ-76) and contain Thr538 or pThr538 in the kinase activation loop. In contrast, PKCθs localised in the Golgi complex have an M r of 85 kDa (θ-85) and, although unphosphorylated at Thr 538, are catalytically active. Strikingly, only θ-76 species which are unphosphorylated at Thr538 can undergo autocatalytic conversion to θ-85. Moreover, a Thr538→Ala PKCθ mutant is constitutively localised in the Golgi complex, confirming that changes in the phosphorylation state of this residue play a pivotal role in the overall control of catalytic properties and localisation of this kinase.",

keywords = "Erythroleukemia cell, Golgi complex, Protein kinase C θ phosphorylation, Thr →Glu protein kinase Cθ, Thr→Ala protein kinase Cθ",

author = "Bianca Sparatore and Mario Passalacqua and Marco Pedrazzi and Sabina Ledda and Mauro Patrone and Deborah Gaggero and Sandro Pontremoli and Edon Melloni",

note = "Funding Information: This work was supported by grants from the Italian Ministero dell{\textquoteright}Istruzione, Universit{\`a} e Ricerca (Cofin 2002), from the University of Genoa and FIRB, Post Genome, Subproject 1. We thank Dr S. Shaw and Dr Y. Liu at the National Cancer Institute, Bethesda, MD, USA for providing pSer 676 and pSer 695 PKCθ antibodies.",

year = "2003",

month = nov,

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doi = "10.1016/S0014-5793(03)01073-1",

language = "English",

volume = "554",

pages = "35--40",

journal = "FEBS Letters",

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TY - JOUR

T1 - Role of the kinase activation loop on protein kinase C θ activity and intracellular localisation

AU - Sparatore, Bianca

AU - Passalacqua, Mario

AU - Pedrazzi, Marco

AU - Ledda, Sabina

AU - Patrone, Mauro

AU - Gaggero, Deborah

AU - Pontremoli, Sandro

AU - Melloni, Edon

N1 - Funding Information: This work was supported by grants from the Italian Ministero dell’Istruzione, Università e Ricerca (Cofin 2002), from the University of Genoa and FIRB, Post Genome, Subproject 1. We thank Dr S. Shaw and Dr Y. Liu at the National Cancer Institute, Bethesda, MD, USA for providing pSer 676 and pSer 695 PKCθ antibodies.

PY - 2003/11/6

Y1 - 2003/11/6

N2 - Multiple protein kinase C (PKC) θ species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCθs localised in the detergent-soluble cell fraction have an Mr of 76 kDa (θ-76) and contain Thr538 or pThr538 in the kinase activation loop. In contrast, PKCθs localised in the Golgi complex have an M r of 85 kDa (θ-85) and, although unphosphorylated at Thr 538, are catalytically active. Strikingly, only θ-76 species which are unphosphorylated at Thr538 can undergo autocatalytic conversion to θ-85. Moreover, a Thr538→Ala PKCθ mutant is constitutively localised in the Golgi complex, confirming that changes in the phosphorylation state of this residue play a pivotal role in the overall control of catalytic properties and localisation of this kinase.

AB - Multiple protein kinase C (PKC) θ species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCθs localised in the detergent-soluble cell fraction have an Mr of 76 kDa (θ-76) and contain Thr538 or pThr538 in the kinase activation loop. In contrast, PKCθs localised in the Golgi complex have an M r of 85 kDa (θ-85) and, although unphosphorylated at Thr 538, are catalytically active. Strikingly, only θ-76 species which are unphosphorylated at Thr538 can undergo autocatalytic conversion to θ-85. Moreover, a Thr538→Ala PKCθ mutant is constitutively localised in the Golgi complex, confirming that changes in the phosphorylation state of this residue play a pivotal role in the overall control of catalytic properties and localisation of this kinase.

KW - Erythroleukemia cell

KW - Golgi complex

KW - Protein kinase C θ phosphorylation

KW - Thr →Glu protein kinase Cθ

KW - Thr→Ala protein kinase Cθ

UR - https://www.scopus.com/pages/publications/0142246600

U2 - 10.1016/S0014-5793(03)01073-1

DO - 10.1016/S0014-5793(03)01073-1

M3 - Article

SN - 0014-5793

VL - 554

SP - 35

EP - 40

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Role of the kinase activation loop on protein kinase C θ activity and intracellular localisation

Abstract

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