Role of BASIC PENTACYSTEINE transcription factors in a subset of cytokinin signaling responses

Shanks CM, A Hecker, Cheng CY, L Brand, SILVIO COLLANI, M Schmid, Schaller GE, D Wanke, K Harter, Kieber JJ

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Cytokinin plays diverse roles in plant growth and development, generally acting by modulating gene tran- scription in target tissues. The type-B Arabidopsis response regulators (ARR) transcription factors have emerged as primary targets of cytokinin signaling and are required for essentially all cytokinin-mediated changes in gene expression. The diversity of cytokinin function is likely imparted by the activity of various transcription factors working with the type-B ARRs to alter specific sets of target genes. One potential set of co-regulators modulating the cytokinin response are the BARLEY B-RECOMBINANT/BASIC PENTACYS- TEINE (BBR/BPC) family of plant-specific transcription factors. Here, we show that disruption of multiple BPCs results in reduced sensitivity to cytokinin. Further, the BPCs are necessary for the induction of a sub- set of genes in response to cytokinin. We identified direct in vivo targets of BPC6 using ChIP-Seq and found an enrichment of promoters of genes differentially expressed in response to cytokinin. Further, a significant number of BPC6 regulated genes are also direct targets of the type-B ARRs. Potential cis-binding elements for a number of other transcription factors linked to cytokinin action are enriched in the BPC binding frag- ments, including those for the cytokinin response factors (CRFs). In addition, several BPCs interact with a subset of type-A ARRs. Consistent with these results, a significant number of genes whose expression is altered in bpc mutant roots are also mis-expressed in crf1,3,5,6 and type-A arr3,4,5,6,7,8,9,15 mutant roots. These results suggest that the BPCs are part of a complex network of transcription factors that are involved in the response to cytokinin.
Lingua originaleInglese
RivistaPlant Journal
DOI
Stato di pubblicazionePubblicato - 2018

Keywords

  • Arabidopsis thaliana
  • ChIP-Seq
  • cytokinin
  • phytohormones
  • transcription factor

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