TY - JOUR
T1 - Renin and angiotensin converting enzyme in elasmobranchs
AU - Uva, B.
AU - Masini, M. A.
AU - Hazon, N.
AU - O'Toole, L. B.
AU - Henderson, I. W.
AU - Ghiani, P.
N1 - Funding Information:
This study was supported by Grant 11/CT87.00245.04/115.15860 from the Italian CNR Joint Research Program.
PY - 1992/6
Y1 - 1992/6
N2 - Renin-like activity (RLA) and angiotensin I converting enzyme-like activity (ACELA), the two key enzymes of the renin-angiotensin system (RAS), were sought in the elasmobranch Scyliorhinus canicula. Renal extracts were desalted in a G-25 and eluted in a G-100 Sephadex column (calibration 15,000-70,000). The fractions were concentrated in a vacuum device. A 48,000-MW fraction incubated with synthetic and porcine angiotensinogen generated angiotensin I estimated by RIA. This same fraction was vasopressor in rats and dogfish. ACELA was sought in gill, heart, liver, spleen, pancreas, intestine, kidney, gonads, brain, skin, and muscle of dogfish using a spectrophotometric assay. The highest level of ACELA was found in the gills followed by spleen, kidney, and brain (33.79 ± 2.3, 29.56 ± 1.0, 14.62 ± 1.0, and 13.80 ± 2.3 nmol hippurate/min/mg protein, respectively). Intestine, gonads, skin and muscle contained no measurable amounts of ACELA. Captopril inhibited enzymatic activity from all ACELA containing tissues.
AB - Renin-like activity (RLA) and angiotensin I converting enzyme-like activity (ACELA), the two key enzymes of the renin-angiotensin system (RAS), were sought in the elasmobranch Scyliorhinus canicula. Renal extracts were desalted in a G-25 and eluted in a G-100 Sephadex column (calibration 15,000-70,000). The fractions were concentrated in a vacuum device. A 48,000-MW fraction incubated with synthetic and porcine angiotensinogen generated angiotensin I estimated by RIA. This same fraction was vasopressor in rats and dogfish. ACELA was sought in gill, heart, liver, spleen, pancreas, intestine, kidney, gonads, brain, skin, and muscle of dogfish using a spectrophotometric assay. The highest level of ACELA was found in the gills followed by spleen, kidney, and brain (33.79 ± 2.3, 29.56 ± 1.0, 14.62 ± 1.0, and 13.80 ± 2.3 nmol hippurate/min/mg protein, respectively). Intestine, gonads, skin and muscle contained no measurable amounts of ACELA. Captopril inhibited enzymatic activity from all ACELA containing tissues.
UR - http://www.scopus.com/inward/record.url?scp=0026748354&partnerID=8YFLogxK
U2 - 10.1016/0016-6480(92)90065-R
DO - 10.1016/0016-6480(92)90065-R
M3 - Article
SN - 0016-6480
VL - 86
SP - 407
EP - 412
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 3
ER -