Rationally designed hyaluronic acid-based nano-complexes for pentamidine delivery

Flavia Carton; Yves Chevalier; Letizia Nicoletti; Małgorzata Tarnowska; Barbara Stella; Silvia Arpicco; Manuela Malatesta; Lars Petter Jordheim; Stéphanie Briançon; Giovanna Lollo

doi:10.1016/j.ijpharm.2019.118526

Rationally designed hyaluronic acid-based nano-complexes for pentamidine delivery

Flavia Carton, Yves Chevalier, Letizia Nicoletti, Małgorzata Tarnowska, Barbara Stella, Silvia Arpicco, Manuela Malatesta, Lars Petter Jordheim, Stéphanie Briançon, Giovanna Lollo

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Nanoparticles of polymeric complexes made of hyaluronic acid and polyarginine were investigated for the encapsulation of the cationic hydrophilic drug pentamidine isethionate. The interaction between the anionic hyaluronic acid and the cationic pentamidine resulting in the formation of polyelectrolyte complexes was firstly studied. Then, nanoparticles made of hyaluronic acid and polyarginine loaded with pentamidine were developed. These drug delivery systems consist of a monodisperse population of negatively charged pentamidine-loaded nanoparticles with a high drug encapsulation rate (80%). Such high encapsulation efficiency coming from ion exchange was confirmed by measurements of the counterion isethionate released from pentamidine during nanoparticles formation. Besides, freeze-dried pentamidine-loaded nanoparticles kept their integrity after their reconstitution in water. In vitro studies on human lung (A549) and breast (MDA-MB-231) cancer cell lines showed that pentamidine-loaded nanoparticles were more cytotoxic in comparison to the free drug, suggesting an enhanced internalization of encapsulated drug by cancer cells.

Lingua originale	Inglese
Numero di articolo	118526
Rivista	International Journal of Pharmaceutics
Volume	568
DOI	https://doi.org/10.1016/j.ijpharm.2019.118526
Stato di pubblicazione	Pubblicato - 10 set 2019
Pubblicato esternamente	Sì

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title = "Rationally designed hyaluronic acid-based nano-complexes for pentamidine delivery",

abstract = "Nanoparticles of polymeric complexes made of hyaluronic acid and polyarginine were investigated for the encapsulation of the cationic hydrophilic drug pentamidine isethionate. The interaction between the anionic hyaluronic acid and the cationic pentamidine resulting in the formation of polyelectrolyte complexes was firstly studied. Then, nanoparticles made of hyaluronic acid and polyarginine loaded with pentamidine were developed. These drug delivery systems consist of a monodisperse population of negatively charged pentamidine-loaded nanoparticles with a high drug encapsulation rate (80%). Such high encapsulation efficiency coming from ion exchange was confirmed by measurements of the counterion isethionate released from pentamidine during nanoparticles formation. Besides, freeze-dried pentamidine-loaded nanoparticles kept their integrity after their reconstitution in water. In vitro studies on human lung (A549) and breast (MDA-MB-231) cancer cell lines showed that pentamidine-loaded nanoparticles were more cytotoxic in comparison to the free drug, suggesting an enhanced internalization of encapsulated drug by cancer cells.",

keywords = "Biomaterials, Drug delivery systems, Hyaluronic acid, Pentamidine, Polyarginine",

author = "Flavia Carton and Yves Chevalier and Letizia Nicoletti and Ma{\l}gorzata Tarnowska and Barbara Stella and Silvia Arpicco and Manuela Malatesta and Jordheim, {Lars Petter} and St{\'e}phanie Brian{\c c}on and Giovanna Lollo",

note = "Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = sep,

day = "10",

doi = "10.1016/j.ijpharm.2019.118526",

language = "English",

volume = "568",

journal = "International Journal of Pharmaceutics",

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TY - JOUR

T1 - Rationally designed hyaluronic acid-based nano-complexes for pentamidine delivery

AU - Carton, Flavia

AU - Chevalier, Yves

AU - Nicoletti, Letizia

AU - Tarnowska, Małgorzata

AU - Stella, Barbara

AU - Arpicco, Silvia

AU - Malatesta, Manuela

AU - Jordheim, Lars Petter

AU - Briançon, Stéphanie

AU - Lollo, Giovanna

PY - 2019/9/10

Y1 - 2019/9/10

N2 - Nanoparticles of polymeric complexes made of hyaluronic acid and polyarginine were investigated for the encapsulation of the cationic hydrophilic drug pentamidine isethionate. The interaction between the anionic hyaluronic acid and the cationic pentamidine resulting in the formation of polyelectrolyte complexes was firstly studied. Then, nanoparticles made of hyaluronic acid and polyarginine loaded with pentamidine were developed. These drug delivery systems consist of a monodisperse population of negatively charged pentamidine-loaded nanoparticles with a high drug encapsulation rate (80%). Such high encapsulation efficiency coming from ion exchange was confirmed by measurements of the counterion isethionate released from pentamidine during nanoparticles formation. Besides, freeze-dried pentamidine-loaded nanoparticles kept their integrity after their reconstitution in water. In vitro studies on human lung (A549) and breast (MDA-MB-231) cancer cell lines showed that pentamidine-loaded nanoparticles were more cytotoxic in comparison to the free drug, suggesting an enhanced internalization of encapsulated drug by cancer cells.

AB - Nanoparticles of polymeric complexes made of hyaluronic acid and polyarginine were investigated for the encapsulation of the cationic hydrophilic drug pentamidine isethionate. The interaction between the anionic hyaluronic acid and the cationic pentamidine resulting in the formation of polyelectrolyte complexes was firstly studied. Then, nanoparticles made of hyaluronic acid and polyarginine loaded with pentamidine were developed. These drug delivery systems consist of a monodisperse population of negatively charged pentamidine-loaded nanoparticles with a high drug encapsulation rate (80%). Such high encapsulation efficiency coming from ion exchange was confirmed by measurements of the counterion isethionate released from pentamidine during nanoparticles formation. Besides, freeze-dried pentamidine-loaded nanoparticles kept their integrity after their reconstitution in water. In vitro studies on human lung (A549) and breast (MDA-MB-231) cancer cell lines showed that pentamidine-loaded nanoparticles were more cytotoxic in comparison to the free drug, suggesting an enhanced internalization of encapsulated drug by cancer cells.

KW - Biomaterials

KW - Drug delivery systems

KW - Hyaluronic acid

KW - Pentamidine

KW - Polyarginine

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U2 - 10.1016/j.ijpharm.2019.118526

DO - 10.1016/j.ijpharm.2019.118526

M3 - Article

SN - 0378-5173

VL - 568

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

M1 - 118526

ER -

Rationally designed hyaluronic acid-based nano-complexes for pentamidine delivery

Abstract

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