PU.1 and CEBPA expression in acute myeloid leukemia

Francesco D'Alò; Annalisa Di Ruscio; Francesco Guidi; Emiliano Fabiani; Mariangela Greco; Carlo Rumi; Stefan Hohaus; Maria Teresa Voso; Giuseppe Leone

doi:10.1016/j.leukres.2008.01.007

PU.1 and CEBPA expression in acute myeloid leukemia

Francesco D'Alò, Annalisa Di Ruscio, Francesco Guidi, Emiliano Fabiani, Mariangela Greco, Carlo Rumi, Stefan Hohaus, Maria Teresa Voso, Giuseppe Leone

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.

Lingua originale	Inglese
pagine (da-a)	1448-1453
Numero di pagine	6
Rivista	Leukemia Research
Volume	32
Numero di pubblicazione	9
DOI	https://doi.org/10.1016/j.leukres.2008.01.007
Stato di pubblicazione	Pubblicato - set 2008
Pubblicato esternamente	Sì

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10.1016/j.leukres.2008.01.007

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title = "PU.1 and CEBPA expression in acute myeloid leukemia",

abstract = "Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.",

keywords = "Acute myeloid leukemia, CEBPA, PU.1, Transcription factors",

author = "Francesco D'Al{\`o} and {Di Ruscio}, Annalisa and Francesco Guidi and Emiliano Fabiani and Mariangela Greco and Carlo Rumi and Stefan Hohaus and Voso, {Maria Teresa} and Giuseppe Leone",

note = "Funding Information: The authors would like to thank Daniel G. Tenen for providing oligonucleotide sequences and excellent suggestions, Iolanda Bayer for cytogenetic data, Pierluigi Puggioni for flow cytometry, and all members of the Laboratory of Molecular Biology at the Institute of Hematology of the Catholic University, Rome. This research was supported by funds from Associazione Italiana per la Ricerca sul Cancro (A.I.R.C.). ",

year = "2008",

month = sep,

doi = "10.1016/j.leukres.2008.01.007",

language = "English",

volume = "32",

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journal = "Leukemia Research",

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TY - JOUR

T1 - PU.1 and CEBPA expression in acute myeloid leukemia

AU - D'Alò, Francesco

AU - Di Ruscio, Annalisa

AU - Guidi, Francesco

AU - Fabiani, Emiliano

AU - Greco, Mariangela

AU - Rumi, Carlo

AU - Hohaus, Stefan

AU - Voso, Maria Teresa

AU - Leone, Giuseppe

N1 - Funding Information: The authors would like to thank Daniel G. Tenen for providing oligonucleotide sequences and excellent suggestions, Iolanda Bayer for cytogenetic data, Pierluigi Puggioni for flow cytometry, and all members of the Laboratory of Molecular Biology at the Institute of Hematology of the Catholic University, Rome. This research was supported by funds from Associazione Italiana per la Ricerca sul Cancro (A.I.R.C.).

PY - 2008/9

Y1 - 2008/9

N2 - Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.

AB - Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.

KW - Acute myeloid leukemia

KW - CEBPA

KW - PU.1

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=43049178362&partnerID=8YFLogxK

U2 - 10.1016/j.leukres.2008.01.007

DO - 10.1016/j.leukres.2008.01.007

M3 - Article

SN - 0145-2126

VL - 32

SP - 1448

EP - 1453

JO - Leukemia Research

JF - Leukemia Research

IS - 9

ER -

PU.1 and CEBPA expression in acute myeloid leukemia

Abstract

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