TY - JOUR
T1 - Proteome and Physiological Characterization of Halotolerant Nodule Endophytes
T2 - The Case of Rahnella aquatilis and Serratia plymuthica
AU - Novello, Giorgia
AU - Gamalero, Elisa
AU - Massa, Nadia
AU - Cesaro, Patrizia
AU - Lingua, Guido
AU - Todeschini, Valeria
AU - Caramaschi, Alice
AU - Favero, Francesco
AU - Corà, Davide
AU - Manfredi, Marcello
AU - Marengo, Emilio
AU - Pelagi, Micaela
AU - Pangaro, Loredana
AU - Caffiero, Giuseppina
AU - Milano, Fulvia
AU - Bona, Elisa
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5
Y1 - 2022/5
N2 - Bacterial endophytes were isolated from nodules of pea and fava bean. The strains were identified and characterized for plant beneficial activities (phosphate solubilization, synthesis of indole acetic acid and siderophores) and salt tolerance. Based on these data, four strains of Rahnella aquatilis and three strains of Serratia plymuthica were selected. To shed light on the mechanisms underlying salt tolerance, the proteome of the two most performant strains (Ra4 and Sp2) grown in the presence or not of salt was characterized. The number of proteins expressed by the endophytes was higher in the presence of salt. The modulated proteome consisted of 302 (100 up-regulated, 202 down-regulated) and 323 (206 up-regulated, 117 down-regulated) proteins in Ra4 and Sp2, respectively. Overall, proteins involved in abiotic stress responses were up-regulated, while those involved in metabolism and flagellum structure were down-regulated. The main up-regulated proteins in Sp2 were thiol: disulfide interchange protein DsbA, required for the sulfur binding formation in periplasmic proteins, while in Ra4 corresponded to the soluble fraction of ABC transporters, having a role in compatible solute uptake. Our results demonstrated a conserved response to salt stress in the two taxonomically related species.
AB - Bacterial endophytes were isolated from nodules of pea and fava bean. The strains were identified and characterized for plant beneficial activities (phosphate solubilization, synthesis of indole acetic acid and siderophores) and salt tolerance. Based on these data, four strains of Rahnella aquatilis and three strains of Serratia plymuthica were selected. To shed light on the mechanisms underlying salt tolerance, the proteome of the two most performant strains (Ra4 and Sp2) grown in the presence or not of salt was characterized. The number of proteins expressed by the endophytes was higher in the presence of salt. The modulated proteome consisted of 302 (100 up-regulated, 202 down-regulated) and 323 (206 up-regulated, 117 down-regulated) proteins in Ra4 and Sp2, respectively. Overall, proteins involved in abiotic stress responses were up-regulated, while those involved in metabolism and flagellum structure were down-regulated. The main up-regulated proteins in Sp2 were thiol: disulfide interchange protein DsbA, required for the sulfur binding formation in periplasmic proteins, while in Ra4 corresponded to the soluble fraction of ABC transporters, having a role in compatible solute uptake. Our results demonstrated a conserved response to salt stress in the two taxonomically related species.
KW - bacterial endophytes
KW - legume nodules
KW - plant beneficial activities
KW - proteome
KW - salt tolerance
UR - https://www.scopus.com/pages/publications/85128860674
U2 - 10.3390/microorganisms10050890
DO - 10.3390/microorganisms10050890
M3 - Article
SN - 2076-2607
VL - 10
JO - Microorganisms
JF - Microorganisms
IS - 5
M1 - 890
ER -