TY - JOUR
T1 - Protection from UVB toxicity in human keratinocytes by thailand native herbs extracts
AU - Thongrakard, Visa
AU - Ruangrungsi, Nijsiri
AU - Ekkapongpisit, Maneerat
AU - Isidoro, Ciro
AU - Tencomnao, Tewin
PY - 2014/1
Y1 - 2014/1
N2 - Thai traditional medicine employs a wide range of indigenous herbs in the forms of tincture or tea for the cure of skin and systemic inflammatory diseases. The protection by Thai plants extracts against UVB DNA damage and cytotoxicity was investigated in human keratinocytes. Petroleum ether, dichloromethane and ethanol extracts were prepared from 15 Thai herb species, and the total phenolic and flavonoid contents, the antioxidant and UV-absorbing properties were assessed by standard procedures. Cytoprotective effects were evaluated on the basis of cell survival, caspase-3 activity and pyrimidine dimers determination. High total phenolic and flavonoid contents were found in the ethanol and dichloromethane fractions. Dichloromethane extract of turmeric was shown to possess the highest antioxidant activity. The maximum UV absorptions were found in the ethanol extract of turmeric and in the dichloromethane extract of ginger. These extracts stimulated the synthesis of Thioredoxin 1, an antioxidant protein, and could protect human HaCaT keratinocytes from UV-induced DNA damage and cytotoxicity. The present data support the utilization of turmeric and ginger extracts in anti-UV cosmetic pharmaceuticals. The protection by Thai plants extracts against UVB DNA damage and cytotoxicity was investigated in human keratinocytes. Ethanol extract of turmeric and dichloromethane extract of ginger were shown to possess the maximum UV absorptions (a). These extracts stimulated the synthesis of Thioredoxin 1, an antioxidant protein (b), and could protect human HaCaT keratinocytes exposed to UVB from DNA damage (d) and caspase-3-mediated apoptosis (c). The present data support the utilization of turmeric and ginger extracts in anti-UV cosmetic pharmaceuticals.
AB - Thai traditional medicine employs a wide range of indigenous herbs in the forms of tincture or tea for the cure of skin and systemic inflammatory diseases. The protection by Thai plants extracts against UVB DNA damage and cytotoxicity was investigated in human keratinocytes. Petroleum ether, dichloromethane and ethanol extracts were prepared from 15 Thai herb species, and the total phenolic and flavonoid contents, the antioxidant and UV-absorbing properties were assessed by standard procedures. Cytoprotective effects were evaluated on the basis of cell survival, caspase-3 activity and pyrimidine dimers determination. High total phenolic and flavonoid contents were found in the ethanol and dichloromethane fractions. Dichloromethane extract of turmeric was shown to possess the highest antioxidant activity. The maximum UV absorptions were found in the ethanol extract of turmeric and in the dichloromethane extract of ginger. These extracts stimulated the synthesis of Thioredoxin 1, an antioxidant protein, and could protect human HaCaT keratinocytes from UV-induced DNA damage and cytotoxicity. The present data support the utilization of turmeric and ginger extracts in anti-UV cosmetic pharmaceuticals. The protection by Thai plants extracts against UVB DNA damage and cytotoxicity was investigated in human keratinocytes. Ethanol extract of turmeric and dichloromethane extract of ginger were shown to possess the maximum UV absorptions (a). These extracts stimulated the synthesis of Thioredoxin 1, an antioxidant protein (b), and could protect human HaCaT keratinocytes exposed to UVB from DNA damage (d) and caspase-3-mediated apoptosis (c). The present data support the utilization of turmeric and ginger extracts in anti-UV cosmetic pharmaceuticals.
UR - http://www.scopus.com/inward/record.url?scp=84891825409&partnerID=8YFLogxK
U2 - 10.1111/php.12153
DO - 10.1111/php.12153
M3 - Article
SN - 0031-8655
VL - 90
SP - 214
EP - 224
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 1
ER -
