Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish

M. A. Masini; M. Sturla; M. Pestarino; F. Facchinetti; A. Gallinelli; B. M. Uva

doi:10.1016/S0196-9781(98)00145-4

Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish

M. A. Masini, M. Sturla, M. Pestarino, F. Facchinetti, A. Gallinelli, B. M. Uva

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Antisera against adrenocorticotropic hormone (ACTH), α-melanocyte stimulating hormone (α-MSH) and β-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones.

Lingua originale	Inglese
pagine (da-a)	87-91
Numero di pagine	5
Rivista	Peptides
Volume	20
Numero di pubblicazione	1
DOI	https://doi.org/10.1016/S0196-9781(98)00145-4
Stato di pubblicazione	Pubblicato - gen 1999
Pubblicato esternamente	Sì

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10.1016/S0196-9781(98)00145-4

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@article{b85c59cf1af64a3a9a9210e7a48c425d,

title = "Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish",

abstract = "Antisera against adrenocorticotropic hormone (ACTH), α-melanocyte stimulating hormone (α-MSH) and β-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones.",

keywords = "Dipnoans, Immunohistochemistry, Lungfish, POMC mRNA, POMC-related peptides, Protopterus annectens, Skin",

author = "Masini, {M. A.} and M. Sturla and M. Pestarino and F. Facchinetti and A. Gallinelli and Uva, {B. M.}",

note = "Funding Information: This research was supported by a grant of the Italian MURST. The authors are indebted to Dr. J. P. H. Burbach (Utrecht, The Netherlands) for the generous gift of the cDNA probe.",

year = "1999",

month = jan,

doi = "10.1016/S0196-9781(98)00145-4",

language = "English",

volume = "20",

pages = "87--91",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "1",

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TY - JOUR

T1 - Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish

AU - Masini, M. A.

AU - Sturla, M.

AU - Pestarino, M.

AU - Facchinetti, F.

AU - Gallinelli, A.

AU - Uva, B. M.

N1 - Funding Information: This research was supported by a grant of the Italian MURST. The authors are indebted to Dr. J. P. H. Burbach (Utrecht, The Netherlands) for the generous gift of the cDNA probe.

PY - 1999/1

Y1 - 1999/1

N2 - Antisera against adrenocorticotropic hormone (ACTH), α-melanocyte stimulating hormone (α-MSH) and β-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones.

AB - Antisera against adrenocorticotropic hormone (ACTH), α-melanocyte stimulating hormone (α-MSH) and β-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones.

KW - Dipnoans

KW - Immunohistochemistry

KW - Lungfish

KW - POMC mRNA

KW - POMC-related peptides

KW - Protopterus annectens

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0032899540&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(98)00145-4

DO - 10.1016/S0196-9781(98)00145-4

M3 - Article

SN - 0196-9781

VL - 20

SP - 87

EP - 91

JO - Peptides

JF - Peptides

IS - 1

ER -

Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish

Abstract

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