TY - JOUR
T1 - Promoter architecture of mouse olfactory receptor genes
AU - Plessy, Charles
AU - Pascarella, Giovanni
AU - Bertin, Nicolas
AU - Akalin, Altuna
AU - Carrieri, Claudia
AU - Vassalli, Anne
AU - Lazarevic, Dejan
AU - Severin, Jessica
AU - Vlachouli, Christina
AU - Simone, Roberto
AU - Faulkner, Geoffrey J.
AU - Kawai, Jun
AU - Daub, Carsten O.
AU - Zucchelli, Silvia
AU - Hayashizaki, Yoshihide
AU - Mombaerts, Peter
AU - Lenhard, Boris
AU - Gustincich, Stefano
AU - Carninci, Piero
PY - 2012/3
Y1 - 2012/3
N2 - Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
AB - Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
UR - http://www.scopus.com/inward/record.url?scp=84857857538&partnerID=8YFLogxK
U2 - 10.1101/gr.126201.111
DO - 10.1101/gr.126201.111
M3 - Article
SN - 1088-9051
VL - 22
SP - 486
EP - 497
JO - Genome Research
JF - Genome Research
IS - 3
ER -