TY - JOUR
T1 - Pro-oxidant processes and antioxidant defence systems in the tissues of the Antarctic scallop (Adamussium colbecki) compared with the Mediterranean scallop (Pecten jacobaeus)
AU - Viarengo, A.
AU - Canesi, L.
AU - Garcia Martinez, P.
AU - Peters, L. D.
AU - Livingstone, D. R.
PY - 1995/5
Y1 - 1995/5
N2 - Antioxidant defences (scavengers, enzymes), pro-oxidant processes (in vitro NAD(P)H-dependent iron/EDTA-mediated hydroxyl radical (·OH) production and susceptibility to lipid peroxidation) were investigated in the tissues of the antarctic scallop Adamussium colbecki (Smith, 1902) compared with a scallop species typical of temperate, mediterranean waters, Pecten jacobaeus L. Levels of free radical scavengers (total glutathione, vitamin E, total carotenoids) and activities of antioxidant enzymes (superoxide dismutase, EC 1.15.1.1.; catalane, EC 1.11.1.6.; glutathione peroxidase, EC 1.11.1.9.) were in the same range in both species and in both were higher in digestive gland than gills. The in vitro potential for -OH production was higher in the digestive gland than the gills for both species, consistent with the former tissue's higher levels of antioxidant defences. Inhibition studies (A. colbecki) indicated the involvement of superoxide anion radical and hydrogen peroxide in ·OH formation. The digestive gland of A. colbecki showed a lower susceptibility to 'in vitro' stimulated lipid peroxidation than that of P. jacobaeus. The results are discussed in relation to the adaptation of A. coibecki to extreme Antarctic conditions, including low temperature, high oxygen tension and marked seasonality of food intake, all of which can influence reactive oxygen species formation.
AB - Antioxidant defences (scavengers, enzymes), pro-oxidant processes (in vitro NAD(P)H-dependent iron/EDTA-mediated hydroxyl radical (·OH) production and susceptibility to lipid peroxidation) were investigated in the tissues of the antarctic scallop Adamussium colbecki (Smith, 1902) compared with a scallop species typical of temperate, mediterranean waters, Pecten jacobaeus L. Levels of free radical scavengers (total glutathione, vitamin E, total carotenoids) and activities of antioxidant enzymes (superoxide dismutase, EC 1.15.1.1.; catalane, EC 1.11.1.6.; glutathione peroxidase, EC 1.11.1.9.) were in the same range in both species and in both were higher in digestive gland than gills. The in vitro potential for -OH production was higher in the digestive gland than the gills for both species, consistent with the former tissue's higher levels of antioxidant defences. Inhibition studies (A. colbecki) indicated the involvement of superoxide anion radical and hydrogen peroxide in ·OH formation. The digestive gland of A. colbecki showed a lower susceptibility to 'in vitro' stimulated lipid peroxidation than that of P. jacobaeus. The results are discussed in relation to the adaptation of A. coibecki to extreme Antarctic conditions, including low temperature, high oxygen tension and marked seasonality of food intake, all of which can influence reactive oxygen species formation.
KW - Antarctic scallop
KW - Antioxidant systems
KW - Low temperature adaptation
KW - Marine invertebrates
KW - Mediterranean scallop
KW - Pro-oxidant processes
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=0028963985&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(94)00228-M
DO - 10.1016/0305-0491(94)00228-M
M3 - Article
SN - 0305-0491
VL - 111
SP - 119
EP - 126
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 1
ER -