TY - JOUR
T1 - Photoactive hybrid nanomaterials
T2 - Indocyanine immobilized in mesoporous MCM-41 for "in-cell" bioimaging
AU - Gianotti, Enrica
AU - Bertolino, Chiara A.
AU - Benzi, Caterina
AU - Nicotra, Giuseppina
AU - Caputo, Giuseppe
AU - Castino, Roberta
AU - Isidoro, Ciro
AU - Coluccia, Salvatore
PY - 2009/3/25
Y1 - 2009/3/25
N2 - Mesoporous silica nanoparticles are being explored as versatile tools for various biomedical and biotechnological applications including disease diagnosis, drug delivery, and intracellular imaging. In this paper, the synthesis and characterization of a fluorescent hybrid mesoporous silica nanomaterial, which is noncytotoxic and shows great potential for "in-cell" bioimaging applications, will be described. The hybrid mesoporous material has been obtained by confining highly fluorescent organic dyes, belonging to the indocyanine family, within the channels of mesoporous MCM-41. To explore the dispersion of the dye inside the mesoporous channels and the formation of dye aggregates, several hybrid samples with increasing dye/MCM-41 loading (up to 100 mg/g) were prepared. A uniform distribution of monomeric 1,1′-diethyl-3,3,3′,3′-tetramethylindocarbocyanine iodide has been achieved at low dye loading (1 mg/g), as evidenced by photoluminescence spectra and lifetime, while a progressive formation of J-aggregates is induced by an increase in the dye loading. To elucidate the properties of the dye immobilized in mesoporous MCM-41, a detailed physical chemical characterization by structural (X-ray diffraction), volumetric and optical (Fourier transform infrared, diffuse-reflectance UV'vis and photoluminescence) techniques has been performed. By ultrasonication of the bulk material, nanoparticles of 2'20 nm diameter were obtained. Biocompatibility, endocytic uptake, and intracellular compartmentalization of such fluorescent nanoparticles were investigated in mammalian cultured cells.
AB - Mesoporous silica nanoparticles are being explored as versatile tools for various biomedical and biotechnological applications including disease diagnosis, drug delivery, and intracellular imaging. In this paper, the synthesis and characterization of a fluorescent hybrid mesoporous silica nanomaterial, which is noncytotoxic and shows great potential for "in-cell" bioimaging applications, will be described. The hybrid mesoporous material has been obtained by confining highly fluorescent organic dyes, belonging to the indocyanine family, within the channels of mesoporous MCM-41. To explore the dispersion of the dye inside the mesoporous channels and the formation of dye aggregates, several hybrid samples with increasing dye/MCM-41 loading (up to 100 mg/g) were prepared. A uniform distribution of monomeric 1,1′-diethyl-3,3,3′,3′-tetramethylindocarbocyanine iodide has been achieved at low dye loading (1 mg/g), as evidenced by photoluminescence spectra and lifetime, while a progressive formation of J-aggregates is induced by an increase in the dye loading. To elucidate the properties of the dye immobilized in mesoporous MCM-41, a detailed physical chemical characterization by structural (X-ray diffraction), volumetric and optical (Fourier transform infrared, diffuse-reflectance UV'vis and photoluminescence) techniques has been performed. By ultrasonication of the bulk material, nanoparticles of 2'20 nm diameter were obtained. Biocompatibility, endocytic uptake, and intracellular compartmentalization of such fluorescent nanoparticles were investigated in mammalian cultured cells.
KW - MCM-41
KW - bioimaging
KW - hybrid materials
KW - indocyanine
KW - lysosomes
KW - photoactive nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=70349132188&partnerID=8YFLogxK
U2 - 10.1021/am800196r
DO - 10.1021/am800196r
M3 - Article
SN - 1944-8244
VL - 1
SP - 678
EP - 687
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 3
ER -