PHARMACOLOGICAL CHARACTERIZATION AND AUTORADIOGRAPHIC LOCALIZATION OF DIHYDROPYRIDINE‐TYPE CALCIUM CHANNELS IN THE KIDNEY OF SPONTANEOUSLY HYPERTENSIVE RATS

Francesco Amenta, Aiping Liu, Maurizio Sabbatini

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Abstract

1. The pharmacological profile and the microanatomical localization of Ca2+ channels of the L‐type were analysed in sections of the kidney of Wistar‐Kyoto (WKY) rats and of spontaneously hypertensive rats (SHR) of different ages. 2. [3H]‐Nicardipine was used as a ligand. It was bound to sections of rat kidney in a manner consistent with the labelling of Ca2+ channels of the L‐type. The density of [3H]‐nicardipine binding sites was similar in WKY rats of different ages and in SHR of 2 and 4 months, but was significantly increased in SHR of 6 months. 3. Light microscope autoradiography revealed the highest density of binding sites in the tubular portion of the nephron and to a lesser extent within smooth muscle of renal arteries and renal corpuscles. In SHR of 4 and 6 months the density of [3H]‐nicardipine binding sites was increased within the epithelium of proximal tubules and of the loop of Henle and decreased in renal corpuscles in comparison with WKY rats or 2 month old SHR. 4. These results show that the density of Ca2+ channels of the L‐type increases with the worsening of hypertension in SHR. The observation of a different sensitivity to hypertension of Ca2+ channels located in the various portions of the nephron indicates the usefulness of light microscope autoradiography for assessing hypertension‐related changes of Ca2+ channels in the kidney.

Lingua originaleInglese
pagine (da-a)S232-S233
RivistaClinical and Experimental Pharmacology and Physiology
Volume22
DOI
Stato di pubblicazionePubblicato - nov 1995
Pubblicato esternamente

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