TY - JOUR
T1 - Perturbations in plant energy homeostasis prime lateral root initiation via SnRK1-bZIP63-ARF19 signaling
AU - Muralidhara, P
AU - Weiste, C
AU - COLLANI, SILVIO
AU - Krischke, M
AU - Kreisz, P
AU - Draken, J
AU - Feil, R
AU - Mair, A
AU - Teige, M
AU - MJ, Müller
AU - Schmid, M
AU - Becker, D
AU - JE, Lunn
AU - Rolland, F
AU - Hanson, J
AU - Dröge-Laser, W
PY - 2021
Y1 - 2021
N2 - Plants adjust their energy metabolism to continuous environmental fluctuations, resulting in a tremendous plasticity in their architec- ture. The regulatory circuits involved, however, remain largely unresolved. In Arabidopsis, moderate perturbations in photosyn- thetic activity, administered by short-term low light exposure or unexpected darkness, lead to increased lateral root (LR) initiation. Consistent with expression of low-energy markers, these treat- ments alter energy homeostasis and reduce sugar availability in roots. Here, we demonstrate that the LR response requires the met- abolic stress sensor kinase Snf1-RELATED-KINASE1 (SnRK1), which phosphorylates the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) that directly binds and activates the promoter of AUXIN RESPONSE FACTOR19 (ARF19), a key regulator of LR initiation. Con- sistently, starvation-induced ARF19 transcription is impaired in bzip63 mutants. This study highlights a positive developmental function of SnRK1. During energy limitation, LRs are initiated and primed for outgrowth upon recovery. Hence, this study provides mechanistic insights into how energy shapes the agronomically im- portant root system.
AB - Plants adjust their energy metabolism to continuous environmental fluctuations, resulting in a tremendous plasticity in their architec- ture. The regulatory circuits involved, however, remain largely unresolved. In Arabidopsis, moderate perturbations in photosyn- thetic activity, administered by short-term low light exposure or unexpected darkness, lead to increased lateral root (LR) initiation. Consistent with expression of low-energy markers, these treat- ments alter energy homeostasis and reduce sugar availability in roots. Here, we demonstrate that the LR response requires the met- abolic stress sensor kinase Snf1-RELATED-KINASE1 (SnRK1), which phosphorylates the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) that directly binds and activates the promoter of AUXIN RESPONSE FACTOR19 (ARF19), a key regulator of LR initiation. Con- sistently, starvation-induced ARF19 transcription is impaired in bzip63 mutants. This study highlights a positive developmental function of SnRK1. During energy limitation, LRs are initiated and primed for outgrowth upon recovery. Hence, this study provides mechanistic insights into how energy shapes the agronomically im- portant root system.
KW - ARF19
KW - Arabidopsis thaliana
KW - SnRK1
KW - bZIP63
KW - root growth
KW - ARF19
KW - Arabidopsis thaliana
KW - SnRK1
KW - bZIP63
KW - root growth
UR - https://iris.uniupo.it/handle/11579/194703
U2 - 10.1073/pnas.2106961118
DO - 10.1073/pnas.2106961118
M3 - Article
SN - 1091-6490
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
ER -