Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing

CHIARA CELESTINA GRASSO; MORENA TREVISAN; VALENTINA FIANO; Valentina Tarallo; Laura De Marco; Carlotta SACERDOTE; RICHIARDI Lorenzo; MERLETTI Franco; Anna Gillio Tos

doi:10.1371/journal.pone.0150483

Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing

CHIARA CELESTINA GRASSO, MORENA TREVISAN, VALENTINA FIANO, Valentina Tarallo, Laura De Marco, Carlotta SACERDOTE, RICHIARDI Lorenzo, MERLETTI Franco, Anna Gillio Tos

Dipartimento di Scienze della Salute

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Pyrosequencing has emerged as an alternative method of nucleic acid sequencing, well suited for many applications which aim to characterize single nucleotide polymorphisms, mutations, microbial types and CpG methylation in the target DNA. The commercially available pyrosequencing systems can harbor two different types of software which allow analysis in AQ or CpG mode, respectively, both widely employed for DNA methylation analysis.

Lingua originale	Inglese
pagine (da-a)	e0150483-e0150483
Numero di pagine	12
Rivista	PLoS ONE
Volume	11
Numero di pubblicazione	3
DOI	https://doi.org/10.1371/journal.pone.0150483
Stato di pubblicazione	Pubblicato - 2016

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10.1371/journal.pone.0150483

https://hdl.handle.net/11579/176734

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abstract = "Pyrosequencing has emerged as an alternative method of nucleic acid sequencing, well suited for many applications which aim to characterize single nucleotide polymorphisms, mutations, microbial types and CpG methylation in the target DNA. The commercially available pyrosequencing systems can harbor two different types of software which allow analysis in AQ or CpG mode, respectively, both widely employed for DNA methylation analysis.",

author = "GRASSO, {CHIARA CELESTINA} and MORENA TREVISAN and VALENTINA FIANO and Valentina Tarallo and {De Marco}, Laura and Carlotta SACERDOTE and RICHIARDI Lorenzo and MERLETTI Franco and {Gillio Tos}, Anna",

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AU - GRASSO, CHIARA CELESTINA

AU - TREVISAN, MORENA

AU - FIANO, VALENTINA

AU - Tarallo, Valentina

AU - De Marco, Laura

AU - SACERDOTE, Carlotta

AU - Lorenzo, RICHIARDI

AU - Franco, MERLETTI

AU - Gillio Tos, Anna

PY - 2016

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AB - Pyrosequencing has emerged as an alternative method of nucleic acid sequencing, well suited for many applications which aim to characterize single nucleotide polymorphisms, mutations, microbial types and CpG methylation in the target DNA. The commercially available pyrosequencing systems can harbor two different types of software which allow analysis in AQ or CpG mode, respectively, both widely employed for DNA methylation analysis.

UR - https://iris.uniupo.it/handle/11579/176734

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DO - 10.1371/journal.pone.0150483

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JO - PLoS ONE

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Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing

Abstract

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