TY - JOUR
T1 - Ovotransferrin supplementation improves the iron absorption
T2 - An in vitro gastro‐intestinal model
AU - Galla, Rebecca
AU - Grisenti, Paride
AU - Farghali, Mahitab
AU - Saccuman, Laura
AU - Ferraboschi, Patrizia
AU - Uberti, Francesca
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Transferrins constitute the most important iron regulation system in vertebrates and some invertebrates. Soluble transferrins, such as bovine lactoferrin and hen egg white ovotransferrin, are glycoproteins with a very similar structure with lobes that complex with iron. In this in vitro study, a comparison of bovine lactoferrin and ovotransferrin was undertaken to confirm the comparability of biological effects. An in vitro gastric barrier model using gastric epithelial cells GTL‐16 and an in vitro intestinal barrier model using CaCo‐2 cells was employed to evaluate iron absorption and barrier integrity. An analysis of the molecular pathways involving DMT‐1 (divalent metal transporter 1), ferritin and ferroportin was also carried out. These in vitro data demonstrate the activity of both 15% saturated and 100% saturated ovotransferrin on the iron regulation system. Compared with the commercial bovine lactoferrin, both 15% saturated and 100% saturated ovotransferrin were found to act in a more physiological manner. Based on these data, it is possible to hypothesise that ovotransferrin may be an excellent candidate for iron supplementation in humans; in particular, 15% saturated ovotransferrin is the overall best performing product. In vivo studies should be performed to confirm this in vitro data.
AB - Transferrins constitute the most important iron regulation system in vertebrates and some invertebrates. Soluble transferrins, such as bovine lactoferrin and hen egg white ovotransferrin, are glycoproteins with a very similar structure with lobes that complex with iron. In this in vitro study, a comparison of bovine lactoferrin and ovotransferrin was undertaken to confirm the comparability of biological effects. An in vitro gastric barrier model using gastric epithelial cells GTL‐16 and an in vitro intestinal barrier model using CaCo‐2 cells was employed to evaluate iron absorption and barrier integrity. An analysis of the molecular pathways involving DMT‐1 (divalent metal transporter 1), ferritin and ferroportin was also carried out. These in vitro data demonstrate the activity of both 15% saturated and 100% saturated ovotransferrin on the iron regulation system. Compared with the commercial bovine lactoferrin, both 15% saturated and 100% saturated ovotransferrin were found to act in a more physiological manner. Based on these data, it is possible to hypothesise that ovotransferrin may be an excellent candidate for iron supplementation in humans; in particular, 15% saturated ovotransferrin is the overall best performing product. In vivo studies should be performed to confirm this in vitro data.
KW - Bovine lactoferrin
KW - Gastro‐intestinal barrier
KW - Iron absorption
KW - Iron metabolism
KW - Iron transportation
KW - Ovotransferrin
UR - http://www.scopus.com/inward/record.url?scp=85118358150&partnerID=8YFLogxK
U2 - 10.3390/biomedicines9111543
DO - 10.3390/biomedicines9111543
M3 - Article
SN - 2227-9059
VL - 9
JO - Biomedicines
JF - Biomedicines
IS - 11
M1 - 1543
ER -